摘要
为考察翘鳞伞胞外多糖对荷瘤小鼠体内肿瘤的抑制及其免疫功能的影响;采用无机陶瓷膜从翘鳞伞(Pholiota squarrosa)AS 5.245液体发酵醪中分离得到的胞外粗多糖(Crude Extracellular Polysaccharide,CEP),以荷瘤S180小鼠为试验对象,用免疫器官重量法,进行高、中、低剂量CEP的腹腔注射试验;试验结果表明,CEP具有较高的抑瘤活性,并呈剂量相关性;中、高剂量组与对照组相比较,小鼠胸腺指数和脾脏指数均有显著增加(P<0.05);与低剂量组相比较,亦均有显著增加;表明CEP可以增强荷瘤小鼠的免疫功能对肿瘤进行抑制。
To investigate the antitumor effect of Pholiota squarrosa polysaeeharide and its immunity regulating effect on S180-bearing mice.An extracellular polysaccharide was extracted from submerged fermentation broth of Pholiota squarrosa AS 5.245 using inorganic ceramic ultrafiltration membrane.With KM mice as animal model,the method of immune organs weight of mice was undertaken.The results showed that the polysaccharide could remarkably inhibit the solid Sarcoma 180 in mice.Furthermore,this effect was dose dependent.Compared with control,medium-and high-dose groups could significantly increase the thymus and spleen index of mice（P〈0.05）,while compared with low-dose group,medium-and high-dose groups could significantly increase the thymus and spleen index of mice,too（P〈0.05）.The suitable concentrations of CEP can enhance the immune functions of mice and play an important role on anti-tumor of S180-bearing mice.
出处
《热带作物学报》
CSCD
2010年第9期1621-1624,共4页
Chinese Journal of Tropical Crops
基金
院级重点科研项目"翘鳞伞胞外多糖膜分离提取及生理活性评价"(2006-201A)资助
关键词
翘鳞伞
胞外多糖
抗肿瘤活性
胸腺指数和脾脏指数
免疫功能
Pholiota squarrosa
Extracellular polysaccharide
Antitumour activity
Thymus and Spleenindex
Immune function
