摘要
目的：对辣椒碱立方液晶凝胶进行表征,建立辣椒碱高效液相含量测定方法。方法：以甘油单油酸酯为液晶材料、辣椒碱为主药,采用自乳化方法制备了辣椒碱立方液晶凝胶;用偏光显微镜及小角衍射对立方液晶凝胶进行了表征;采用高效液相测定了立方液晶凝胶中辣椒碱的含量。结果：立方液晶凝胶在偏光显微镜下视野黑暗;小角衍射显示在0.109 6,0.133 4,0.155 7,0.188 3-1处出现信号峰,峰位置符合q1∶q2∶q3∶q4=2∶3∶4∶6,特征峰显示其内部结构为Pn3m（Q224）。高效液相色谱法测定立方液晶凝胶中的辣椒碱线性范围为3.25×10-4~2.08×10-2g.L-1（R2=1）,平均回收率97.53%,RSD 2.9%（n=9）。结论：偏光显微镜及小角衍射适宜于立方液晶凝胶的表征,高效液相测定方法简便、可行。
Objective： To prepare the cubic phase gel containing capsaicin and characterize its properties.Method： The cubic phases gel composed of glycerol monoolein,capsaicin and water was made by self-emulsion technology.The characterization of cubic phase gel was carried out by Cross-Polarizing light microscopy（CPLM） and Small Angle X-Ray Scattering（SAXS）.The capsaicin content was determined by HPLC analysis.Result： Under CPLM,cubic phase gel showed dark background.SAXS scattering spectra showed the scattering peaks at 0.109 6,0.133 4,0.155 7,0.188 3 -1 which was compatible with q1∶q2∶q3∶q4=2∶3∶4∶6.It was well known that the scattering vector ratio was the characteristic of cubic phase and the internal structure was confirmed to be Pn3m（Q224）.The linear range for capsaicin determination was 3.25×10-4-2.08×10-2g·L-1（R2=1）.The average recovery was 97.53% with RSD of 2.9%（n=9）.Conclusion： CPLM and SAXS technology are suitable to characterize the cubic phase gel.The determination of the capsaicin content by HPLC is simple and reproducible.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第23期3123-3126,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81001643)
广东省东莞市科技计划项目(2008108101064)
广东省中医药局(2009237)
关键词
立方液晶凝胶
辣椒碱
小角X衍射
含量测定
高效液相
cubic phase gel
capsaicin
small angle X-ray scattering（SAXS）
content determination
HPLC
