摘要
目的探讨危重型甲型H1N1流感患者外周血白细胞和T淋巴细胞亚群的变化及其临床意义。方法检测18例危重型甲型H1N1流感患者(危重组),18例普通型H1N1流感患者(普通组)和18例健康人(对照组)外周血白细胞计数及分类,流式细胞术测上述患者外周血T淋巴细胞各亚群CD3+、CD4+、CD8+、CD8+CD28+、CD8+CD28-、CD4+CD25+high的百分比。结果与对照组比较,甲型H1N1病毒感染患者,尤其是危重型组,淋巴细胞绝对数及百分比显著下降,且外周血T淋巴细胞各亚群CD3+、CD4+、CD8+、CD8+CD28+、CD4+CD25+high百分比明显降低,而CD8+CD28-百分比明显增高(P<0.05)。结论甲型H1N1流感病毒感染损伤患者淋巴细胞和细胞免疫功能。外周血淋巴细胞及T淋巴细胞各亚群的百分比改变可反映甲型H1N1流感患者病情的严重程度。
Objective To investigate the changes and clinical significance of white blood cell and T lymphocyte subsets in peripheral blood of critical patients with H1N1 influenza virus infection.Methods The leukocytes and T lymphocyte subsets in peripheral blood were detected in 18 critically illed H1N1 influenza patients（group A）,18 common H1N1 influenza patients（group B） and 18 healthy people（group C） using flow cytometry.Results Compared to group C,the percentages and counts of lymphocytes were significantly lower in groups of A and B,especially in group A.The percentages of CD3^＋,CD4^＋,CD8^＋,CD8^＋CD28^＋ and CD4^＋CD25＋high were remarkably lower,but CD8^＋CD28^-was higher,in group A than those in groups of B and C（P〈0.05）.ConclusionInfection of influenza H1N1 virus damages the lymphocytes and cellular immune function of the patients.The percentages of lymphocytes and T lymphocyte subsets could be used as the parameters in evaluating the severity of the patients with influenza H1N1 virus infection.
出处
《江苏医药》
CAS
CSCD
北大核心
2010年第20期2391-2393,共3页
Jiangsu Medical Journal
