摘要
用限制性内切酶ＥｃｏＲＩ、ＸａｂＩ、ＸｈｏＩ、ＢａｍＨＩ、ＰｓｔＩ、ＳａｃＩ、ＨｉｄｎⅢ、Ｓｍａｌ酶解斜纹夜蛾核多角体病毒广州株基因组ＤＮＡ，分别得到２６、２６、２４、２０、１３、１７、９、１条片段，并算得基因组平均大小为１３６．０ｋｂｐ。以ＡｃＮＰＶ多角体基因的部分读码框为探针，经Ｓｏｕｔｈｅｍ杂交将ＳＩＮＰＶ多角体基因定位于ＸｂａＩＯ片段上。将此片段克隆并序列分析，结果表明ＳＩＮＰＶ的多角体基因位于ＸｂａＩ Ｏ片段的ＸｂａＩ－ＳａｌＩ ０．８ｋｂｐ片段上，其开放读码框为７５０ｂｐ，编码一２４９ａａ的蛋白质，与ＳｐｌｉＮＰＶ相同，为目前所发现的最长的多角体基因。ＳＩＮＰＶ多角体蛋白Ｍｒ＝２９２３６，其氨基酸序列与其它核多角体病毒的多角体蛋白氨基酸序列有高度的同源性。
Digestion of Spodoptera litura nuclear polyhedrosis virus (SINPV Guangzhou isolate) with restrictionendonucleases EcoRI.XbaI.Xhol.Bam HI Pst I SacI .Hind and SmaI resulted in 26.26.24.20. 13. 17.9 and 1fragments, respectively. The average size of the genome is about 136.OKbp. The polyhedrin gene of SINPV waslocated on the Xba I O fragments by southem hybridization methed using a DIG labeled AcMNPV polyhedringene as probe. With a coding sequence of 780 nucleotides, corresponding to a protein of 249 amino acid, thepolyhedrin gene of SINPV is the longest polyhedrin gene known to date together with the Spodoptera littoralisNPV polyhedrin gene. This gene codes for a putative protein with an Mr 29236 and the predicted amino acidsequence shows high homology with those of other NPVs.
出处
《微生物学通报》
CAS
CSCD
北大核心
1999年第2期88-92,共5页
Microbiology China
基金
国家自然科学基金!No.39730030
关键词
斜纹夜蛾
核型多角体病毒
限制性内酶
图谱
Spodoptera litura Nuclear Polyhedrosis Virus, Restriction endonuclease patterns, Polyhedrin gene The nucleotide sequence of polyhedrin gene of SINPV has been submitted to the GeneBank/EMBL Data libraries under the accession number AF 037262
