摘要
用羊β-乳球蛋白基因(BLG)5区5×103b(103b,旧称kb)为调控序列,构建了乳腺表达组织纤溶酶原激活剂突变体(La-tPA)载体.对540枚小鼠受精卵进行显微注射,经PCR和Southernblot检测,获得6只整合有人La-tPA的转基因小鼠,整合率为32%.
The vector of expression La tPA in mammary gland of transgenic animal was constructed by using BLG gens 5 control region.6 transgenic mice were produced by microinjection method.Foreign gene intergation rate was 32% in mice.It provided us some information and methods to produce foreign protein in livestocks.
出处
《生命科学研究》
CAS
CSCD
1999年第1期75-78,共4页
Life Science Research
基金
国家"八六三"项目