摘要
目的:检测新疆维吾尔族人群β2-肾上腺素能受体(β2-AR)基因5′-调控区SNPs,并探讨这些SNPs与新疆维吾尔族EH的关系。方法:应用MALDI-TOF-MS方法测定β2-AR基因5′-调控区-654位与-1429位SNP及类型,并进行基因分型。结果:β2-AR基因5′-调控区-654位与-1429位SNP分别为-654位G→A、-1429位T→A碱基变异。其中-654位SNPs基因型GG、GA、AA频率在正常血压和EH人群间的分布差异无统计学意义(P>0.05),位于-1429位SNPs基因型在两组人群中分布差异无统计学意义(P>0.05)。结论:β2-AR基因-654位与-1429位SNPs可能仅为基因多态性标志。
Objective：To detect single nucleotide polymorphisms（SNPs） existing in 5′-regulatory region of β2-adrenoceptor（β2-AR） gene and to investigate association of the identified SNPs with essential hypertension in Uighur of Xinjiang.Methods：Single nucleotide polymorphisms（SNPs）in the position-654G/A and-1429T/A of β2-AR gene were tested with MALDI-TOF-MS and Genotypes of the SNP were typed by MassARRAY iPLEXTMmethod.Results：Two SNPs were identified at position-654 with G→A substitution and-1429 with T→A substitution.The frequency of genotype of the two SNPs complied well with the Hardy-Weinberg equilibrium in normal group Distribution of genotype AA,GA,GG of the SNPs at locus-654 in hypertension group was no significantly different from that in normal group（P0.05）,No significant difference was observed in distribution of geno-types of the SNPs at locus-1429 between the two groups（P0.05）.Conclusion：These results indicate that the SNPs at locus-654 and-1429 of β2-AR gene were the SNPs position but not linked to hypertension.
出处
《农垦医学》
2010年第3期193-195,共3页
Journal of Nongken Medicine
