摘要
[目的]乳腺癌CD44+/CD24-具有干细胞肿瘤浸润的特点。CD44+/CD24-肿瘤干细胞是否可调节Twist而增加肿瘤转移的风险尚不明确。[方法]培养人类乳腺细胞系MCF7,并注射到裸鼠;同时收集92例乳腺癌患者的标本。用免疫组织化学双染色分析CD44和CD24阳性表达,RT-PCR检测转移标本的Twist表达。[结果]在MCF7细胞系存在CD44+/CD24-细胞。在动物体内,转移组织的CD44+/CD24-细胞更多,Twist高表达。在人类标本,20.7%(19/92)的标本存在CD44+/CD24-细胞,与ER、PR及Her-2的表达无明显联系(r=0.452)。在人体转移癌组织,CD44+/CD24-细胞与原发肿瘤无明显差异,但Twist高表达。[结论]MCF7细胞和人乳腺癌存在CD44+/CD24-细胞。在动物模型中,乳腺癌干细胞可能通过上调Twist表达从而参与肿瘤转移。
[Purpose] Breast cancer cells with a CD44^＋/CD24^-phenotype have been suggested to have tumor-initiating properties with stem cell-like. It is not yet known whether CD44/CD24 phenotype up-regulate Twist gene and increase micrometasis risk. [Methods] The MCF7 breast cancer cell line was cultured by mammosphere and implanted into node mice. Double-staining immunohistochemistry was used to quantify CD44 and CD24 expression in cytospin and tissue samples from animal model and 92 cases with breast tumor. Twist gene expression were examined by RT-PCR. [Results] The cells marked with CD44^＋/CD24^-existed in cell line MCF-7. In animal model,the samples from metastasis tissues had more CD44^＋/CD24^-cells and stronger expression for Twist. In human tumor samples,20.7% contained cells with CD44 ＋/CD24^-phenotype. The CD44^＋/CD24^-phenotype was no relationship with estrogen and progesterone receptors as well as HER2（r =0.452） . In the samples from human metastasis tissues,Twist was over-expression. [Conclusions] MCF-7 breast cancer cell line and human cancer tissue exist CD44^＋/CD24^-cells. Breast cancer stem cells involve distant metastasis through up-regulating the Twist expression in animal model.
出处
《肿瘤学杂志》
CAS
2010年第9期710-714,共5页
Journal of Chinese Oncology
基金
湖北省卫生厅青年科技人才基金(QJX2005-13)
