摘要
目的回顾本实验室开展间接免疫荧光法抗核抗体(ANA)与免疫印迹法特异性抗核抗体谱(ANAs)联合检测的情况,分析两者之间的相关性,并探讨联合检测在自身免疫性疾病中的应用价值。方法采用间接免疫荧光法(IIF)检测1150例血清标本ANAs,线性免疫印迹法(LIA)检测其中217例血清标本ANAs,并分析两种方法检测结果的相互关系。结果 448例男性患者中,阳性率39.7%(178/448);702例女性患者中,阳性率66.1%(464/702),男女两组阳性率有显著差异(χ2=77.08,P<0.05)。217例血清标本以LIA检测ANAs,结果显示:在30例荧光法ANA阴性中,印迹法抗ANAs抗体有4例阳性,阳性率为13.3%;在187例荧光法ANA阳性中,印迹法ANAs有103例阳性,阳性率为55.4%。结论抗核抗体的IIF法筛选易导致以抗SSA、抗SSB和抗Ro52为主要抗体患者的ANA假阴性,而LIA法特异性ANAs的检测因检测的抗体不全面也无法取代ANA的IIF法检测。在临床实际工作中两种ANA的检测方法不能相互取代,应联合应用。
Objective Retrospective analysis on the rsults of anti-nuclear antibodies（ANA） using the indirect immunofluorescence（IIF） and the line immunoassay for specific ANAs, the correlation＇between these two techniques,and the potential application of it in the diagnoses of autoimmune,and the specific ANAs in 217 serum were examined by LIA for anti-nRNP/Sm,anti-Sm, anti-SS-A, anti-Ro-52, anti-SS-B, anti-SCL 70, anti PM-Scl, anti-Jo-1, anti-centromere B, anti-nukleosome, anti-PCNA, anti-ds-DNA, anti-histones,anti-ribosomal-P protein and AMA-M2. The association between the results based on these two techniques was analyzed. Methods Results The positive rate of ANA was 39.7%（178/448） in men,and 66.6%（464/702） in women. It was statistically higher in female than in the male（x^2= 77.08, P〈0.05）. The specific ANAs results of 217 serum showed that, of the 30 patients with negative ANA by IIF,4 of them were positive for specific ANAs by LIA, the positive rate was 13. 3%（4/30） ;Of the 187 cases with positive ANA by IIF,103 of them were positive for specific ANAs by LIA,the positive rate was 55.4%103/187）. Conclusion The IIF was probable to generate a false negative results of ANA when the major autoantibodies are anti-SSA,anti-SSB and anti-Ro52,whereas the LIA is unable to take place of IIF due to limited of specific autoantibodies. Thus, these two techniques should be jointly used in clinical practice.
出处
《国际检验医学杂志》
CAS
2010年第11期1212-1214,1217,共4页
International Journal of Laboratory Medicine
基金
威海市科技发展计划项目(2008093-8)
关键词
抗体
抗核
荧光
核型分析
实验室技术和方法
antibodies,antinuclear
fluorescence
karyotyping
laboratory techniques and procedures
