摘要
目的评估16SrRNA基因检测在自发性细菌性腹膜炎(SBP)快速诊断中的应用价值。方法采用16SrRNA基因荧光定量多聚酶链反应检测76例疑似SBP及6例非感染性腹腔积液患者腹水细菌DNA,并与腹水细菌培养结果进行比较。结果 16S rRNA检测疑似SBP患者腹水标本的阳性率为22.4%,明显高于腹水细菌培养的7.9%(P<0.01)。6例非感染性腹腔积液患者的腹水16SrRNA基因荧光定量PCR检测结果和细菌培养结果均为阴性。结论 16SrRNA检测可做为SBP的快速诊断方法,其敏感性优于腹水细菌培养。
Objective To evaluate the value of ascitic bacterial 16S rRNA gene determination in the rapid diagnosis of spontaneous bacterial peritonitis（SBP）.Methods 16S rRNA gene from bacterial DNA in ascites was determined by quantitative fluorescent polymerase chain reaction（PCR）in 76 patients with suspected SBP and 6 patients with non-infectious ascites.The results were compared with those obtained from bacterial culture.Results The positive rate of SBP was 22.4% among patients detected with ascitic bacterial 16S rRNA gene determination-based quantitative fluorescent PCR,which was significantly higher than that（7.9%）in patients only received bacterial culture（P〈 0.05）.In addition,in 6 patients with non-infectious ascites,both the 16S rRNA gene determination-based quantitative fluorescent PCR and bacterial culture showed negative results.Conclusions 16S rRNA gene determination-based quantitative fluorescent PCR can be an effective tool for the rapid diagnosis of SBP.It is more sensitive than the bacterial culture.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
2010年第5期557-560,共4页
Acta Academiae Medicinae Sinicae
基金
杭州市科技发展计划重点项目(2005633Q02)~~
