摘要
背景与目的:胃癌的发生、发展及生物学行为受到多个分子信号通路的影响,转录因子是调控这些分子通路的重要因素。而转录因子KLF4(Krüppel-like factors 4)、KLF5(Krüppel-like factors 5)与胃癌相关性的报道比较罕见。因此探讨转录因子KLF4、KLF5对胃癌生物学行为影响的分子机制具有重要的意义。方法:收集158例手术切除的胃溃疡型腺癌组织标本,同时取78例癌旁组织作为对照。应用免疫组织化学SP法检测胃腺癌组织、癌旁组织中转录因子KLF4和KLF5蛋白的表达。结果:158例胃腺癌组织中KLF4的阳性表达率为40.50%,KLF5的阳性表达率为63.93%,KLF4和KLF5的表达与患者性别、年龄及肿瘤位置之间无显著相关性(P>0.05),与肿瘤有无淋巴转移及WHO临床分期之间有显著相关性(P<0.05)。随着临床分期等级的增加,KLF4表达呈现递减趋势,而KLF5表达呈现增加趋势,其表达率与肿瘤临床分期程度显著相关(P<0.05)。结论:胃癌组织中KLF4的低表达和KLF5蛋白高表达可能是胃黏膜恶性转变以及胃癌发生浸润转移的重要生物学标志,检测KLF4和KLF5对预测胃癌浸润转移有重要意义。
Background and purpose：The gastric cancer development and biological behavior is affected by a number of elements of signaling pathways,and transcription factors are important element in these regulating molecules.The reports of transcription factor Krüppel-like factors 4（KLF4）,Krüppel-like factors 5（KLF5） reports of gastric cancer was rare.So the research of the transcription factor KLF4,KLF5 biological behavior in gastric cancer molecular mechanisms is very significance.Methods：The expression of the KLF4 and KLF5 were detected by immunohistochemical SP method in 158 case of gastric carcinoma tissue and 78 case of the para-carcinoma mucosa.Results：The positive rate of the KLF4 in gastric carcinoma was 40.50%,and the KLF5 was 63.93%,the KLF4/KLF5 expressions were not significantly corrective with the gender,age,the histology type（P0.05）,however,it corrected with the lymph node metastasis and clinical stage（P0.05）.Conclusion：The KLF4 and KLF5 protein may be biological markers in gastric cancer,and the low expression of KLF4 and the high expression of KLF5 protein in gastric cancer may be important for malignant transformation in gastric cancer invasion and metastasis,and it is important significance to detect KLF4 and KLF5 in gastric cancer to predict the invasion and metastasis.
出处
《中国癌症杂志》
CAS
CSCD
北大核心
2010年第10期756-759,共4页
China Oncology
