摘要
本研究旨在阐明双侧大脑皮层扣带回前部(anterior cingulate gyrus,ACG)在内脏痛觉感受中的作用。实验用强电流刺激一侧内脏大神经(greater splanchnic nerve,GSN)作为内脏伤害性刺激,用在体微电极细胞内电位记录技术记录、分析ACG内神经元对刺激的反应和自发生物电活动。根据对GSN刺激是否发生反应,将ACG内神经元分为产生诱发反应的GSN刺激相关神经元(GSN-stimulus-relative neurons,GSRNs)与不产生诱发反应的非相关神经元。根据诱发反应的潜伏期等性质,GSRNs进一步分为内脏伤害感受神经元(visceral nociceptive neurons,VNNs)与非伤害感受神经元(non-visceral nocicep-tive neurons,NVNNs);而VNNs又分为特异性(specific visceronociceptive neurons,SVNNs)与非特异性内脏伤害感受神经元(non-specific visceronociceptive neurons,NSVNNs)。结果显示,对侧ACG内GSRNs比例为38.18%,显著高于同侧ACG内GSRNs比例(29.49%,P<0.01),表明GSN传入纤维虽然投射到双侧ACG,但主要投射到对侧ACG。和同侧ACG相比,对侧ACG的SVNNs比例较低,而NSVNNs比例则较高(P<0.01)。同侧ACG内GSRNs、VNNs、NVNNs和SVNNs的静息电位(restingpotential,RP)绝对值均显著低于对侧ACG的对应神经元(P<0.01),而双侧ACG内NSVNNs的RP绝对值没有明显差异。双侧ACG内VNNs、NVNNs的自发生物电活动形式、自发放电频率和幅值之间无明显差别;VNNs中具有自发生物电活动的神经元比例显著高于NVNNs中的比例。以上结果提示双侧ACG皮层虽然都能感受GSN的伤害性刺激信号,但双侧ACG皮层的反应模式、反应程度不完全相同,从而为双侧脑功能的不对称性提供了新的科学实验依据。
The aim of the present study is to explore the role of anterior cingulate gyrus（ACG） in bilateral cerebral cortex in visceral nociceptive sensation.Electrical stimulation of greater splanchnic nerve（GSN） was used as visceral nociceptive stimulus,and intracellular recording techniques in vivo was used to record and analyze the responses to stimuli and spontaneous electric activities of the neurons in the bilateral ACG.According to the responses to electrical stimulation of GSN,the neurons in the bilateral ACG were divided into GSN-stimulus-relative neurons（GSRNs） and GSN-stimulus-irrelative ones.According to the characteristics of the evoked responses to electrical stimulation of the GSN,GSRNs could be further classified into visceral nociceptive neurons（VNNs） and nonvisceral nociceptive neurons（NVNNs）.VNNs included specific visceral nociceptive neurons（SVNNs） and non-specific visceral nociceptive neurons（NSVNNs）.The results showed that the percentage of GSRNs in the contralateral ACG（38.18%） was signifi-cantly higher than that in the ipsilateral ACG（29.49%,P0.01）,suggesting although GSN afferent fibers project to bilateral ACG,they mainly project to the contralateral ACG.Compared with ipsilateral ACG,contralateral ACG possessed lower proportion of SVNNs and higher proportion of NSVNNs（P0.01）.The absolute values of resting potentials（RP） of GSRNs,VNNs,NVNNs and SVNNs in ipsilateral ACG were less than those of corresponding neurons in contralateral ACG.However,there were no significant differences in the absolute values of RP of NSVNNs between ipsilateral and contralateral ACG.There were no significant differences in modes,frequencies and amplitudes of spontaneous electric activities of VNNs and NVNNs between ipsilateral and contralateral ACG.Additionally,the percentage of neurons having spontaneous electric activities from VNNs was significantly higher than that from NVNNs,which indicated that the excitability of VNNs was higher than that of the NVNNs in bilateral ACG.Th
出处
《生理学报》
CAS
CSCD
北大核心
2010年第5期450-454,共5页
Acta Physiologica Sinica
基金
supported by the Science and Technology Foundation of Education Department of Liaoning Province
China(No.2023101067)
the Science and Technology Foundation of Department of Science and Technology
Shenyang Municipality
China(No.1022043-1-03
F10-205-1-42)
关键词
扣带回前部
内脏痛
伤害感受神经元
细胞内电位
自发生物电活动
anterior cingulate gyrus
visceral pain
nociceptive neuron
intracellular potential
spontaneous biological electric activity
