摘要
目的 研究hIL-10基因修饰的骨髓间充质干细胞（MSCs）对大鼠原位异种肝移植排斥反应的影响及机制.方法 采用豚鼠对Wistar大鼠的非协调性异种原位肝脏移植模型,实验动物随机分为3组：空白对照组,MSCs组,hIL-10-MSCs组.观察受体存活时间、受体鼠肝功能变化.术后第24小时取移植肝脏,用RT-PCR、ELISA法观察E-Selectin、LFA-1、VCAM-1及NF-κB的表达情况.结果 与空白对照组相比,hIL-10-MSCs组大鼠生存时间延长、肝功能好转,黏附分子E-Selec-tin、LFA-1、VCAM-1和NF-κB表达明显降低（P＜0.05）.结论 hIL-10-MSCs对移植肝脏保护作用可能与其抑制NF-κB、E-Selectin、LFA-1、VCAM-1的表达有关.
Objective To observe the influence of hIL-10-MSCs on rejection of rat discordant liver xenotransplantation. Methods The model of cavia to rat discordant liver xenotransplantation was established. The rats were randomized into the control group, MSCs group, and hIL-10-MSCs group.The survival time and liver function of each recipient were observed and expression levels of E-Selectin, LFA-1, VCAM-1, and NF-κB determined by RT-PCR and ELISA 24 h after transplantation.Results The survival time was prolonged, liver function improved, and expressions levels of E-Selectin, LFA-1, VCAM-1 and NF-κB were significantly decreased in the hIL-10-MSCs group （P〈0.05）.Conclusion hIL-10-MSCs are able to protect transplanted liver and decrease the expression of E-Selectin, LFA-1, VCAM-1, and NF-κB in the liver.
出处
《中华肝胆外科杂志》
CAS
CSCD
北大核心
2010年第10期777-780,共4页
Chinese Journal of Hepatobiliary Surgery
关键词
肝移植
排斥反应
间充质干细胞
免疫耐受
Liver transplantation
Rejection
Mesenchymal stem cell
Immune tolerance
