摘要
目的:试验研究了以二氧化硅粒子为载体固定辣根过氧化物酶的条件及固定化酶酶学特性。方法:通过溶胶-凝胶法制备了一种新的球形二氧化硅粒子并在其表面引入氨基,用戊二醛共价交联法固定化辣根过氧化物酶。分析了戊二醛的浓度、pH值、给酶量等因素对固定化酶活性的影响。结果:最佳固定条件是,戊二醛的溶度在3%(v/v),pH值在7.5,辣根过氧化物酶与二氧化硅微米颗粒的质量比为3.0∶50。固定化酶和游离酶反应的最佳pH值分别为7.5和7.0,最佳反应温度为50℃和40℃。结论:与游离酶相比,固定化酶具有优良的热稳定性和储存稳定性。
Objective：To explore the immobilization conditions using spherical silica particle as carrier and the characteristics of immobilized enzyme.Methods： A new spherical silica particle was prepared by sol-gel method and amino groups were produced on surface of the spherical silica particle.Horseradish peroxidase was immobilized on spherical silica particle with glutaraldehyde by covalent cross-linking reaction.The effect of glutaraldehyde concentration,pH and amount of the enzyme on the enzymatic activity were analyzed.Results： The optimal immobilized were determined： concentration of glutaraldehyde in 3%（v/v）,pH value of 7.5 and 3.0 mg horseradish peroxidase per 50 mg silica particles.The optimal reaction pH value of immobilized enzyme and free enzyme were 7.5 and 7.0 while the reaction temperatures were 50℃ and 40℃,respectively.Conclusion： The immobilized enzyme has good thermal stability and storage stability compared with free enzyme.
出处
《中国卫生检验杂志》
CAS
2010年第11期2769-2771,共3页
Chinese Journal of Health Laboratory Technology
