摘要
目的：观察低分子量肝素（ｌｏｗｍｏｌｅｃｕｌａｒｗｅｉｇｈｔｈｅｐａｒｉｎ，ＬＭＷＨ）对兔结膜成纤维细胞增殖的抑制作用，探讨其与地塞米松联合应用的价值，并与肝素的抑制作用相比较。方法：应用ＭＴＴ法，测定不同浓度的ＬＭＷＨ对结膜成纤维细胞增殖的抑制作用，同时比较单独应用ＬＭＷＨ、地塞米松、同一抗凝单位的肝素及ＬＭＷＨ联合地塞米松对成纤维细胞增殖的抑制作用。结果：ＬＭＷＨ对兔结膜成纤维细胞增殖的抑制作用与浓度成正相关关系，与对照组相比本实验所采用浓度的各种药物均对兔结膜成纤维细胞的增殖有抑制作用（Ｐ＜００５），ＬＭＷＨ比肝素的抑制作用强，差异有显著性（Ｐ＜００５），而与ＬＭＷＨ联合地塞米松相比抑制作用无显著性差异（Ｐ＞００５）。结论：ＬＭＷＨ有可能克服肝素易引起出血的副作用。
To investigate the inhibiting effects of low molecular weight
heparin(LMWH)on conjunctival fibroblast proliferation alone and in combination with
dexamethasone,the anti proliferation effects of heparin were compared also.Using MTT
method,we detected the dose effect relationship of LMWH on inhibiting conjunctival
fibroblast proliferation;we also evaluated the inhibiting effect on rabbit conjunctival
fibroblast proliferation of LMWH alone,LMWH combined dexamethasone and the same
anticoagulative unit heparin.The proliferation of rabbit conjuntival fibroblasts was inhibited
effectively by LMWH,the inhibiting rates changed with concertrations positively.All
medicine inhibited rabbit conjunctival fibroblast proliferation significantly at the tested
concentration( P <0 05).The inhibiting effect of LMWH was higher than that of heparin at
the same anticoagulative unit( P <0 05),no significant difference between LMWH or
dexamethasone alone and the combinition( P >0 05).This study offered a baseline for
further clinic investigation,LMWH may be used to treat proliferative eye diseases.
出处
《眼科》
CAS
1999年第2期117-119,共3页
Ophthalmology in China
