摘要
目的:研究肠易激综合征(Irritable bowel syndrome,IBS)模型大鼠结肠组织5-羟色胺(5-hydroxytryptamine,5-HT)含量、5-HT转化率以及眼针治疗的影响。方法:实验动物分成对照组、IBS模型组、眼针组,采用束缚刺激与应激刺激相结合的方法建立IBS动物模型并进行眼针治疗。各组采用ELISA法检测结肠组织5-HT和5-羟吲哚乙酸(5-hydroxyindoleacetic acid,5-HIAA)的表达,采用RT-PCR法检测结肠组织5-HT重摄取转运体(serotonin reuptaketransporter,SERT)、色氨酸羟化酶1(Tryptophan hydroxylase1,TPH1)的mRNA表达。结果:与对照组比较,IBS模型组5-HT、5-HIAA水平显著升高,5-HT转化率也显著升高,SERT mRNA表达下调而TPH1mRNA表达上调;与IBS模型组比较,眼针组5-HT和5-HIAA水平明显下降,5-HT转化率显著降低,SERT mRNA表达上调而TPH1mRNA表达下调。结论:5-HT降解与合成分泌的不相匹配可能是IBS模型大鼠5-HT水平升高的重要原因,眼针治疗可有效改善IBS大鼠5-HT代谢,使5-HT恢复至正常水平。
Objective：To investigate colonic 5-HT content and percent conversion in the rat with IBS and effects of ocular acupuncture therapy（ OAT）. Methods：Animals were classified into control？？IBS model and OAT groups. Rat model of IBS was established by method of stress and combining bound,and OAT was given. Colonic 5 -HT and 5 -HIAA of each group were examined by ELISA. SERTmRNA and TPH1mRNA were examined by RT -PCR. Results：Compared with those in control,the levels of 5-HT and 5-HIAA increased,percent conversion of 5-HT increased too,expression of SERTmRNA decreased and TPH1mRNA expression increased in IBS model. Compared with those in IBS model,the levels of 5 -HT and 5 -HIAA and percent conversion of 5-HT decreased,expression of SERTmRNA increased and TPH1mRNA expression decreased in OAT. Conclusion：The difference of 5 -HT between anabolic metabolism and catabolism in IBS model suggestes that the disharmony is involved in the pathogenesis of IBS. OAT can correct 5-HT metabolism effectively.
出处
《辽宁中医杂志》
CAS
北大核心
2010年第11期2222-2224,共3页
Liaoning Journal of Traditional Chinese Medicine
基金
国家重点基础研究发展计划(973计划)资助项目(2007CB512702)
关键词
肠易激综合征
5-羟色胺
5-羟吲哚乙酸
眼针
irritable bowel syndrome 5-hydroxytryptamine tryptophan hydroxylase1 serotonin reuptake transporter ocular acupuncture therapy
