摘要
目的研究磷酸肌醇-3激酶/蛋白激酶B(PI3K/PKB)特异性抑制剂LY294002对人脑胶质瘤细胞株U251增殖的影响。方法采用免疫细胞化学S-P法检测LY294002对U251细胞磷酸化蛋白激酶B(P-PKB)蛋白的表达,噻唑蓝(MTT)法检测LY294002(LY2940025、10、20、40μmol·L-1)干预24、48、72h后U251细胞增殖的抑制率。结果 LY294002以剂量、时间依赖性方式抑制U251细胞的生长,LY294002组中不同剂量的干预组与对照组相比P-PKB蛋白的表达降低,差别均有统计学意义(P<0.05);LY294002组中随着剂量的增加,其对U251细胞生长抑制率增加,不同剂量干预组间比较差别有统计学意义(P<0.01)。结论 LY294002对人脑胶质瘤U251细胞有抑制生长的作用。LY294002对P-PKB表达的抑制可能是细胞增殖受抑的重要原因。
Objective To investigate the effect of LY294002 for the proliferation of U251 cell line as a specific inhibitor of phosphoinositide 3-kinase /protein kinase B （ PI3K /PKB）. Methods The phosphorylated protein B （ P-PKB） expression of U251 cell interfered with LY294002 were detected by immunocytochemistry. The inhibition ratio of U251 cell proliferation interfered with LY294002（ 5,10,20,40 ？？ol？？L -1 ） were detected by methyl thiazolyl tetrazolium after intervention 24,48,72 hours. Results The growth of U251 cell was inhibited by LY294002 in dose-dependent and time-dependent way. Compared to the control group,the P-PKB expression in groups interfered with different dose LY294002 decreased（ P 0. 05）. With the increase of the dose of the LY294002,the inhibition ratio of U251 cell proliferation increased,and there was significant difference（ P 0. 01）. Conclusion LY294002 can inhibit the growth of U251 cell of human brain glioma. The inhibition of LY294002 for the expression of P-PKB may play an important role in the suppression of cell proliferation.
出处
《新乡医学院学报》
CAS
2010年第6期566-568,共3页
Journal of Xinxiang Medical University
