摘要
讨论了共焦扫描光学显微镜的高分辨率性质，指出共焦扫描显微镜由于采用点探测器，因而视场大大减小，信噪比大大提高，同时每幅图像逐点扫描形成，在光学系统信息能力不变的前提下，系统的空间域通带宽度增加和时域通带宽度减小。因而可成高分辨率的像，特别是其独特的深度分辨率特性使得可以实现光学断层扫描成像。
Based on the concept of the invariance of the information capacity of an optical system, the property of the high resolution of the confocal scanning optical microscope is discussed. Because a point source and a point detector are used in a confocal microscope, the system is characterized by its very small field of view and high signal to noise ratio and has the decreased temporal bandwidth and increased spatial bandwidth. Therefore a high resolution image would be formed. The optical sectioning images obtained with the confocal fluorescent scanning microscope designed are given.
出处
《光学技术》
EI
CAS
CSCD
1999年第3期4-7,共4页
Optical Technique
关键词
高分辨率
信息能力
荧光显微镜
共焦扫描
high resolution
confocal microscope
information capacity of the optical system
