摘要
以菠菜(Spinacia oleracea L.)为试材,研究了不同浓度毒死蜱对菠菜抗氧化酶活性、酸性磷酸酯酶(APase)活性、丙二醛(MDA)含量、相对电导率以及农药残留量的影响。高浓度毒死蜱对菠菜SOD活性具有明显的抑制作用,除施药后第1d、14 d有上升外,其他时期均明显低于对照组。药后4 h,喷施高浓度毒死蜱的CAT活性高于对照组,但1d后CAT活性与对照组相当。药后4 h,POD的活性明显受到抑制,且药后第21 d喷药处理菠菜的POD活性均低于对照组,表明毒死蜱对菠菜POD的影响时间较长,且总体上其浓度越高影响越大。喷药处理后,菠菜的MDA含量明显高于对照组,且随着毒死蜱浓度的增大而增大。喷施毒死蜱可明显抑制APase的活力,且总体上毒死蜱的浓度越高抑制效果越明显,而胁迫后期的抑制效果逐渐减小。由农药残留量可以看出,总体上喷施毒死蜱浓度越高,其半衰期就越长。防治菠菜虫害时,在保证效果的前提下应尽量减少毒死蜱用量,以减少对菠菜的伤害,提高其食用安全性。
This paper is to investigate the effects of different concentrations of chlorpyrifos spray on the antioxidant enzymes and APase activities, malondiadehyde content, relative conductivity and pesti- cide residue in the spinach plants. The results of our investigation show that different concentrations of the chlorpyrifos spray can indeed bring about different effects on the activities of the antioxidant enzymes. That is to say, high concentration of chlorpyrifos spray tends to inhibit the activity of SOD. At 4 h after the spray, the plants would give a stronger response to the activity of CAT on the condition of higher concentration of chlorpyrifos spray than the contrast. The POD activity in the spinach would be inhibited at 4 h after the pesti- cide spray. Until 21 d after the treatments, the activity of POD seems likely to be lower than the contrast. This result indicates that such ef- fects on the POD activity tend to be longer than other antioxidant en- zymes, moreover, the effect proves greater with the increase of con- centration of chlorpyrifos totally. In addition, it has proved that dif- ferent concentrations of chlorpyrifos are likely to help to increase the content of MDA in the plant. The activity of APase would be obvious- ly inhibited with the increase of concentration of chlorpyrifos totally. It was not until the later period of pesticide stress, the inhibition of chlorpyrifos began to be gradually decreased. Thus, the determination result of the pesticide residue in the spinach shows the half-life of the chlorpyrifos turns to be longer with the increase of chlorpyfifos concentration. Therefore, when using pesticides to prevent spinach from insect pest, we should try to reduce the dosage of chlorpyrifos, with a view to minimising the damage of chlorpyrifos in spinach, and improving edible security of spinach.
出处
《安全与环境学报》
CAS
CSCD
北大核心
2010年第5期6-10,共5页
Journal of Safety and Environment
基金
青岛市自然科学基金项目(07-2-3-4-jch)
关键词
植物保护学
毒死蜱
胁迫
抗氧化酶
丙二醛
菠菜
plant protection
chlorpyrifos
stress
antioxidant enzyme
malondiadehyde （MDA）
spinach
