摘要
以正常绒毛为对照,通过qRT-PCR检测自然流产胚胎绒毛组织中hsa-miR-26a的表达水平;利用基因表达谱芯片筛选自然流产胚胎绒毛的差异表达基因,并使用Targetscan预测这些差异表达基因中可能为hsa-miR-26a所调控的靶基因.结果显示hsa-miR-26a在自然流产胚胎绒毛中表达上调,差异表达基因中hsa-miR-26a可能调控的靶基因有2个:白血病抑制因子(LIF)和结缔组织生长因子(CTGF).hsa-miR-26a可能通过转录后调控着床相关基因LIF的表达,进而调节HCG水平,影响妊娠结局.
With normal villi as the control, the expression level of hsa-miR-26a in spontaneous abortion villi was detected by qRT-PCR and differentially expressed genes in spontaneous abortion villi were analyzed by cDNA microarray. Among these differentially expressed genes, target genes regulated by hsa-miR-26a were predicted with Targetscan. The result showed that the expression level of hsa-miR-26a in spontaneous abortion villi was significantly higher than that of normal villi and the target genes regulated by hsa-miR-26a may involve the leukemia inhibitory factor （LIF） and the connective tissue growth factor （CT- GF）. The observation suggests that hsa-miR-26a might influence the expression of LIF by post-transcriptional control, thereby regulating the level of HCG and resulting in abortion.
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第10期59-64,共6页
Journal of Southwest University(Natural Science Edition)
基金
国家自然科学基金资助项目(30700898)
重庆市自然科学基金资助项目(CSTC
2007BB5286)
