摘要
从猪＂高热病＂病料中分离到一株猪繁殖与呼吸综合征病毒（SD-TA株）,该病毒能够被抗PRRSV N蛋白单克隆抗体所识别。根据GenBank PRRSV经典株（VR-2332）和变异株（JXA1）基因序列分别设计合成了10对引物,利用RT-PCR扩增其基因序列,分别得到了预期的基因片段,将扩增的cDNA片段克隆入pMD18-T载体并测序。应用DNASTAR软件分析,与国内外已发表毒株的序列进行比较。结果显示,与美洲型相比,PRRSV SD-TA株相应基因核苷酸同源性为89.3%-99.1%,并且在Nsp2上缺失了30个氨基酸;与欧洲型代表毒株LV株相比同源性为59.9%。遗传关系表明：SD-TA株属于高致病性变异株。
In 2008, one PRRSV （SD-TA strain）was isolated from the ＇ Ardent Fever Disease＇ in pigs which could react with the monoclonal antibodies against N proteins. The ten pairs of primers were designed respectively as the gene sequences of PRRSV classical strain （ VR- 2332） and mutational strain （JXA1） from GenBank and the aimed gene fragments were amplified respectively by RT-PCR. Then amplified cDNA fragments were cloned into the vector pMD18-T and sequenced. The sequences were analyzed compared with the sequences from the strains in and aboard the country by the software DNA Star. The results showed that the nucleotides homologies were 89.3 % - 99.1% between PRRSV SD-TA strain and American strain and 30 nucleotides in the fragments of Nsp2 were deleted. Compared with European strain LV, the nucleotides homologies were 59.9 %. The genetic relationships showed that SD-TA strain was the highly pathogenic PRRSV strain.
出处
《西南农业学报》
CSCD
北大核心
2010年第5期1698-1702,共5页
Southwest China Journal of Agricultural Sciences
基金
国家自然科学基金项目(30871857)
山东省自主创新成果转化重大专项项目(2008ZHX1A1101)
