摘要
从蛋白质角度对水分胁迫下甘蔗苗期根系蛋白质SDS-PAGE电泳图谱的变化进行研究,以揭示水分胁迫下甘蔗苗期根系蛋白质的表达差异。甘蔗品种新台糖16号砂培育苗至苗期茎高约25 cm进行处理,将幼苗根系放入含100 g/L聚乙二醇6000的Hoagland营养液中进行模拟干旱胁迫处理,处理不同时间段采取根样品,SDS-PAGE电泳分析法探讨水分胁迫对供试甘蔗品种根系蛋白质变化与水分胁迫强度的关系。与对照相比,在水分胁迫处理的24 h内,稳定出现的新诱导合成蛋白条带分子量为14.2KDa蛋白;对胁迫响应最快的是81.7和52.6 KDa蛋白条带,胁迫开始2 h就显著下调;而33.8 KDa蛋白条带在胁迫后相当长时间保持稳定,后期才显著上调;60.3、26.1、16.2 KDa蛋白条带胁迫后期变化活跃;PEG胁迫5 h处理蛋白质条带变化最大,既有新增蛋白条带(88.7、65.9、31.9、14.2 KDa)与消失蛋白条带(64.9 KDa),又有上调表达蛋白条带(60.3 KDa)与下调表达蛋白条带(81.7、52.6、26.1 KDa)。甘蔗根系蛋白质在响应PEG胁迫的基因表达调控中扮演重要角色,上述蛋白条带的变化可能与甘蔗水分胁迫密切相关。
The present study was carried out to investigate the effect of drought stress on the differential protein expression in sugarcane roots by SDS-PAGE. The plants of the sugarcane variety ROC16 were used as the experimental materials, which were cultivated in sand bed. Sugarcane plants were treated with nutrient solution containing 10 % （W/V） polyethylene glycol 6000 （PEG 6000 ） for simulating drought stress at their 25 cm height. The proteins from different samples were separated by SDS-PAGE. Compared with the control, the stable exist- ence of the new protein band was 14.2 KDa protein during 24 h PEG stress, which may be the main new regulatory protein responding drought stress. The fastest responding protein bands were 81.7 and 52.6 KDa ones, which decreased markedly at the early PEG stress stage of 2 h. But the relative content of 33.8 KDa protein band remained stable for a long time before declining at the late PEG stress stage. The relative content of 60.3,26.1 and 16.2 KDa protein bands changed actively during the late PEG stress. The protein bands showed the most at 5 h of PEG stress. Four new protein bands （88.7, 65.9, 31.9 and 14.2 KDa） were found and a protein band （64.9 KDa） was disap- peared. Meanwhile, the protein bands of 81.7,52.6 and 26.1 KDa were down-regulated while the 60.3 KDa protein band was up-regulated markedly in roots at 5 h of PEG stress. Proteins in sugarcane roots play an important role in gene expression and regulation, and their chan- ges should be related with the occurrence of drought stress.
出处
《西南农业学报》
CSCD
北大核心
2010年第5期1455-1459,共5页
Southwest China Journal of Agricultural Sciences
基金
国家科技支撑计划项目(2007BAD30B03)
广西科技攻关重点项目(桂科攻0782004-3)
广西科技攻关项目(桂科能0815011)
现代农业产业技术体系建设专项项目(nycytx-024-01-15)
关键词
甘蔗
根
水分胁迫
差异蛋白
SDS-PAGE
Sugarcane
Roots
Drought stress
Differential expression proteins
SDS-PAGE
