摘要
目的:了解EB病毒(Epstein-Barr virus,EBV)相关胃癌(EBVaGC)和阴性胃癌(EBVnGC)组织中视网膜母细胞瘤(Rb)基因甲基化状态及蛋白表达,探讨EBV感染与Rb基因甲基化的关系。方法:采用甲基化特异性PCR(MSP)对各种临床病理指标匹配的23例EBVaGC和25例EBVnGC组织及相应癌旁组织中Rb基因启动子区域的甲基化状态进行检测,并采用免疫组化技术检测两种胃癌组织中Rb蛋白的表达。结果:胃癌与相应癌旁组织中Rb基因启动子区的甲基化率分别为64.6%(31/48)和39.6%(19/48),差异有显著性(P<0.05);EBVaGC组织中Rb基因启动子区的甲基化率为82.6%(19/23),高于EBVnGC中的检出率(48.0%,12/25),差异有显著性(P<0.05);EBVaGC和EBVnGC组织中Rb蛋白的表达率分别为52.2%(12/23)和72.0%(18/25),差异无显著性(P﹥0.05);胃癌组织中Rb启动子基因甲基化与蛋白表达无明显负相关。结论:Rb异常甲基化在胃癌细胞中较常见,EBV可以诱导Rb基因甲基化,影响其基因表达而参与EBVaGC的发生。
Objective： To understand the promoter methylation status of retinoblastoma （Rb） gene and its expression at protein level in Epstein-Barr virus （EBV）-positive and-negative gastric carcinomas, and to investigate the correlation of EBV with Rb methylation and expression. Methods： A series of 23 EBV-associated gastric carcinoma （EBVaGC） and 25 matched EBV-negative gastric carcinoma （EBVnGC） tissues were examined for the promoter methylation of Rb by methylation-specific PCR method. The expression of Rb was detected by immunohistochemistry. Results： The methylation rate of Rb gene was 64.6% （31/48） in carcinoma tissue, 39.6%（19/48） in para-carcinoma tissue, 82.6%（19/23）in EBVaGC tissue and 48.0% （12/25） in EBVnGC tissue, respectively. The difference of methylation rate between carcinoma and para-carcinoma was significant （P〈0.05）, and also between EBVaGC and EBVnGC （P〈0.05）. There was no significant difference for Rb expression between EBVaGC （52.0%, 13/23） and EBVnGC （72.0%, 18/25）. Rb methylation was not significantly correlates with Rb expression. Conclusions： Methylation of Rb is a common event in gastric carcinomas and EBV induces hypermethylation of Rb in EBVaGC, which may contribute to the development of gastric carcinomas.
出处
《现代生物医学进展》
CAS
2010年第20期3834-3837,3861,共5页
Progress in Modern Biomedicine
基金
青岛市科技局资助项目(No08-2-1-4-nsh)
