期刊文献+

静态光散射表征蛋白质-蛋白质相互作用:抑制动力学和解离

Characterizing Protein-Protein Interactions Via Static Light Scattering: Inhibition Kinetics and Dissociation
下载PDF
导出
摘要 通过加入小分子抑制物,时间依赖多角度静态光散射(TD-MALS)可以用于表征蛋白质相互作用抑制动力学而无需采用标记、固载、底物或其他修饰方法。该方法可以同时测出蛋白质-蛋白质亲和力。 Time-dependent multiangle, static light scattering (TD-MALS) is used to determine the inhibition kinetics of protein interactions by a small-molecule inhibitor, without tagging, immobilization, substrates, or other modifications. The method can simultaneously indicate protein-protein binding affinity.
出处 《生命科学仪器》 2010年第5期26-29,共4页 Life Science Instruments
  • 相关文献

参考文献10

  • 1Some D, Hanlon A, Sockolov K. Characterizing protein -protein interactions via static light scattering: reversible heteroassociation. Am Biotech Lab, 2008, 26(4): 18-20. 被引量:1
  • 2Flamig D P, Parkhurst L J. Kinetics of the alkaline tetramer - dimer dissociation in liganded human hemoglobin: a laser light-scattering stopped - flow study. Proc Natl Acad Sci, U.S.A. 1977, 74(9):3814-6. 被引量:1
  • 3Gorisch H, Goss D J, Parkhurst L J. Kinetics of ribosome dissociation and subunit assoeiation studied in a light- scattering stopped-fow apparatus. Biochemistry, 1976, 15(26): 5743-53. 被引量:1
  • 4Lyles D S, McKenzie M O, Hantgan R R. Stopped-flow, classical, and dynamic light-scaltering analysis of matrix protein binding to nucleocapsids of vesticular stomatitis virus. Biochemistry, 1996, 35(20): 6508-18. 被引量:1
  • 5Lai E, van Zanten J H. Monitoring DNA/poly-l-lysine polyplex formation with time-resolved muhiangle laser light scattering. Biophys J, 2001, 80(2): 864-73. 被引量:1
  • 6Bernocco S, Ferri F, Profumo A, Cuniberti C, Rocco M. Polymerization of rod-like macromolecular monomers studied by stopped-flow, multiangle light scattering: set-up, data processing and application to fbrin formation. Biophys J, 2000, 79(1): 561-83. 被引量:1
  • 7Gilleland M J, Bender M L. Kinetics of chymotrypsin dimerization. J Biol Chem, 1976, 251(2): 498-502,. 被引量:1
  • 8Mintz G R. An irreversible serine protease inhibitor. Biopharm, 1993, 6(2): 34-8. 被引量:1
  • 9Kameyama K, Minton A P. Rapid quantitative characterization of protein interactions by composition gradient static light scattering. Biophys J, 2006, 90(6): 2164-9. 被引量:1
  • 10Some D, Berges A, Ferrullo J, Hitchner E, Yang J. TD-MALS characterization of antibody-antigen interaction kinetics. Intl Light Scattering Coil, Oct 20-21, 2008, Santa Barbara, CA. 被引量:1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部