摘要
目的：研究长穗桑茎皮中具有抗炎和细胞毒活性的化学成分。方法：采用各种柱色谱对长穗桑茎皮95%乙醇提取物的醋酸乙酯部位进行分离纯化,通过NMR,MS等波谱分析手段鉴定化合物结构。结果：从醋酸乙酯部位中分离得到9个酚类化合物,其中6个黄酮类化合物,3个二苯乙烯类化合物。分别鉴定为槲皮素（1）,5,7,3,′4′-四羟基-3-甲氧基黄酮（2）,norartocarpanone（3）,二氢山柰酚（4）,euchrenone a7（5）,morachalcone A（6）,白藜芦醇（7）,高白藜芦醇（8）,4′-异戊烯基高白藜芦醇（9）。化合物18分别进行了抗炎和细胞毒活性筛选。结论：化合物19均为首次从该植物中分离得到。其中化合物6和8浓度在1.0×10^-5mol.L-1时对血小板活化因子刺激的多形核白细胞β-葡萄糖苷酸酶释放的抑制率分别为76.8%,94.2%;化合物2和8分别对人卵巢癌细胞（A2780）和人胃癌细胞（BGC-823）有选择性细胞毒活性,IC50分别为0.66,1.31μmol.L^-1。
To search for the chemical constituents possessing anti-inflammatory or cytotoxic activities from plants,Morus wittiorum was investigated for the first time.The stem bark of M.wittiorum was extracted with 95% EtOH.The EtOH extract was fractionationed on silica gel by eluting with petroleum ehter,CHCl3 and EtOAc successively.The further isolation and purification of the EtOAc fraction of 95% EtOH extract was performed by various column chromatography such as silica gel,Sephadex LH-20,RP-C18 column chromatography and so on.The structures of compounds were determined on the basis of spectral analysis such as NMR,MS etc.As a result,nine compounds were isolated including six flavonoids and three stilbenoids and elucidated as quercetin（1）,5,7,3′,4′-tetrahydroxy-3-methoxyflavone（2）,norartocarpanone（3）,dihydrokaempferol（4）,euchrenone a7（5）,morachalcone A（6）,resveratrol（7）,oxyresveratrol（8）,4′-prenyloxyresveratrol（9）.Compounds 1-9 were isolated from this plant for the first time,among which compounds 1-8 were evaluated for their anti-inflammatory and cytotoxic activities,respectively.Wherein compounds 6 and 8 showed inhibition to the release of β-glucuronidase from rat polymorphonuclear leukocyte（PMNs） induced by platelet activating factor（PAF） at a concentration of 10-5 mol·L-1.The inhibitory ratios were 76.8%,94.2% individually.Compounds 2 and 8 exhibited selective cytotoxicity agaist human ovarian cancer（A2780） and human gastric cancer（BGC-823） with IC50 values as 0.66,1.31 μmol·L^-1 individually.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第20期2700-2703,共4页
China Journal of Chinese Materia Medica
基金
国家科技重大专项综合平台项目(2009ZX09301-003-12-1)
关键词
长穗桑
黄酮类
二苯乙烯类
抗炎
细胞毒
Morus wittiorum
flavonoids
stilbenoids
anti-inflammation
cytotoxicity
