摘要
利用Ｈ２Ｏ２易通过细胞膜而到达核这一特点，初步探讨了不同浓度Ｈ２Ｏ２对ＨＬ６０细胞ＤＮＡ的氧化损伤程度．发现Ｈ２Ｏ２浓度在０４ｍｍｏｌ／Ｌ以上时，作用８～２４ｈ可以用气相色谱／火焰离子检测器（ＧＣ／ＦＩＤ）检测到氧化损伤标志产物———８羟基鸟嘌呤（８ｏｈＧ），并观测到在０４～０８ｍｍｏｌ／ＬＨ２Ｏ２作用一定时间时。
Although hydrogen peroxide is generated in cells by normal metabolism, the reaction of the stress or by treatment of the cells with 0 4 mmol/L of hydrogen peroxide for 8～24 hours can result in DNA damage. 8 hydroxyguanine is a typical hydroxyl radical induced products in DNA.The yields of the 8 hydroxyguanine in DNA isolated from H 2O 2 treated HL 60 cells in different ways were reported.The 8 hydroxyguanine in the cells treated in more than 0 4 mmol/L H 2O 2 for eight hours.In order to identify the 8 hydroxyguanine, the result was confirmed by CGC/MS SIM. The results suggested that the nuclear DNA damage in cells caused by H 2O 2 may be involved in formation of hydroxyl radicals.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1999年第3期280-283,共4页
Progress In Biochemistry and Biophysics
基金
广东省重点学科资助课题
关键词
8-羟基鸟嘌呤
H2O2
GC/FID
细胞氧化损伤
human promyeloxytic leukemia, 8 hydroxyguanine , H 2O 2, gas chromatography/flame ion detector
