摘要
采用正交实验设计,以美洲商陆浆果总黄酮提取率为考察指标,对影响总黄酮提取率的各类因素进行了研究,初步确定提取美洲商陆浆果中总黄酮较佳的工艺条件,并测定了总黄酮的含量。结果表明,美洲商陆浆果中总黄酮提取的较佳工艺条件为:水浴温度为60℃,乙醇浓度为60%,提取时间为60min,固液比为1:40,总黄酮(以芦丁计)得率为3.12%。
The optimization of extraction method of total favonoids from the fruits of Phytolacca americana L. was obtained by using orthogonal test design, and the content of total flavonoids (rutin as a comparetion) was determined, the results showed that 60℃ of water--bath temperature, 1 : 40 of ratio of solid to ethanol solution and 60min extraction time were the optimal conditions, under which ratio of total flavonoids (rutin as a comparetion ) in fruits of Phytolacca americana L. was 3.12%.