摘要
目的了解P2X7受体在离体培养的少突胶质前体细胞(OPCs)缺氧缺血性损伤中的作用。方法建立离体OPCs培养模型,通过乳酸脱氢酶(LDH)释放百分比评估细胞死亡;Western blot分析缺氧缺血前后OPCs P2X7受体表达变化。结果 (1)缺氧缺糖(OGD)后2 h,近40%OPCs死亡。OGD前预先给予P2X7受体拮抗剂BBG起到部分保护作用;OGD条件下,P2X7受体激动剂BzATP加重OPCs缺氧缺血性损伤,并且这种增强的毒性作用不能被BBG拮抗。(2)Western blot显示,OGD后2 h,P2X7受体蛋白表达迅速下调。结论 P2X7受体可能参与了OPCs缺氧缺血性损伤过程。
Objective To observe the role of P2X7 receptor in hypoxic-ischemic damage to oligodendrocyte precuresor cells（OPCs） in vitro.Methods To cultivate,retrieve and purify oligodendrocyte progenitor cells（OPCs） from neonatal rats.LDH released in the culture was used to assess the cell death quantitatively;Western blot was used to measure the change of P2X7 receptor expression.Results（1）LDH release assays showed that 2 h oxygen-glucose deprivation（OGD） exposure killed near 40% of the cells by 2 h.P2X7 receptor antagonist,BBG applied before OGD partially protected OPCs from OGD-induced cell death.Furthermore,P2X7R agonist BzATP exacerbated OGD induced OPCs injury,and the enhancement in toxicity could not be prevented by BBG.（2）Immunoblots revealed that P2X7R protein was significantly decreased in OPCs cultures compared with controls at 2 h after OGD.Conclusion P2X7R involves in the process of hypoxic-ischemic white matter injury.
出处
《重庆医学》
CAS
CSCD
北大核心
2010年第21期2895-2897,共3页
Chongqing medicine
基金
国家自然科学基金资助项目(30900467)
