摘要
目的研究脑外伤后脑水肿、缺血性脑损害的发生与循环内皮细胞（CEC）、内皮素（ET）及内皮素A受体（ETAR）变化的关系。方法建立颅脑撞击伤的动物模型，于伤后不同时相测定CEC、血、CSF、不同脑区ET含量，原位杂交检测ETARmRNA表达，测定脑血流量、脑含水量，光镜、电镜观察神经病理学改变。结果脑外伤后CEC、ET含量显著升高，ETARmRNA表达增强，并与伤情轻重呈正相关。结论脑外伤后脑血管内皮细胞脱落致血脑屏障（BBB）破坏，升高的ET与ETAR结合使血管通透性增高，脑血流减少，参与了脑水肿、缺血性脑损害的发生。
Objective The present study was designed to investigate the correlation of the development of brain edema and ischemic brain damage with the changes o0f circulating endothelial cells(CEC), endothelin(ET) and endothelin-A receptor(ETAR) in rabbits following traumatic brain injury(TBI ). Methods By using a brain impact model in rabbits, the changes of CEC, cerebral blood flow(CBF) and brain water content were measured. the concentration of ET in plasma, cerebral spine fluid(CSF) and differently regional brain tissue was studied at certain time postinjury.Meanwhile the expression ETAR-mRNA was determined by the methods of in situ hybridization. The pathomorphological changes of the injured brain tissue were observed. The levels of CEC and ET were also observed. Results The levels of CEC, ET and expression of ETAR-mRNA increased significantly after TBI, which correlated well with the degree of brain injury. Conclusion These findings indicate that the drop of CEC leads to the disruption of the brain-blood barrier(BBB). The combination of ET and ETAR results in the elevation of permeability and decline of CBF, both giving rise to the development of brain edema and ischemic brain insult.
出处
《创伤外科杂志》
1999年第1期4-7,共4页
Journal of Traumatic Surgery
关键词
颅脑损伤
CEC
ET
ETAR
继发性
脑损害
脑水肿
Brain injury Circulating endothelial cell Endothelin Endothelin receptor Secondary brain injury
