摘要
目的:建立益生胶囊的质量控制方法。方法:采用薄层色谱法对黄芪、女贞子、淫羊藿、补骨脂进行定性色谱鉴别;采用双波长薄层扫描法测定黄芪甲苷的含量,以三氯甲烷-甲醇-水(13∶7∶2)下层溶液为展开剂,10%硫酸乙醇溶液显色,λ_s=530 nm,λ_R=700 nm。结果:黄芪、女贞子、补骨脂、淫羊藿的定性鉴别专属性强;黄芪甲苷点样量在2.588～7.764μg范围内线性关系良好,r=0.997 7,平均加样回收率为99.70%(RSD=3.26%,n=6)。结论:本方法定性定量准确、可靠,可用于控制益生胶囊的质量。
Objective： To establish a quality control standard for yisheng capsules. Method： Radix astragali,Fructus ligustri,luci- di,Herba epimedii, Fructus psoraleae were identified by TLC, and the content of Astragaloside IV was determined by TLC-scanning. Resuit： The sports in the TLC were clear and distinguished well. The linear range of Astragaloside IV was from 2. 588 μg to 7. 764 μg,r =0. 997 7. The average recovery rates were 99. 70% ,with the RSD was 3.26% （n =6）. Conclusion： This method is reliable and accurate,it can be used for the quality control of yishcng capsules.
出处
《中国药师》
CAS
2010年第11期1548-1550,共3页
China Pharmacist
基金
全军医药卫生科研基金(2006182006)
关键词
益生胶囊
黄芪甲苷
薄层扫描
含量测定
Yisheng capsules
Astragaloside IV
TLC-scanning
Content determination
