摘要
目的研究实验性牙移动初期大鼠牙周组织中CD133+血管内皮祖细胞(EPC)的表达。方法 40只Wistar大鼠,随机分为实验组和对照组,建立牙移动动物模型。于加力后1、3、5、7、14 d处死动物并制作以上颌第一磨牙为中心近远中向的组织切片,进行苏木精-伊红染色、CD133免疫组织化学染色。结果大鼠正常牙周组织中未见CD133表达的新生血管。实验性牙移动初期,实验组牙周组织新生微血管内皮细胞中可见CD133阳性染色,牙周组织CD133表达于加力后1 d达到高峰,此后逐渐下降,但与对照组相比差异无统计学意义(P>0.05)。结论 CD133+EPC参与了实验性牙移动牙周组织早期的血管改建,但其直接参与牙周组织血管生成较少,可能大多通过旁分泌作用参与新血管的形成。
Objective To investigate the expression patterns of CD133＋ endothelial progenitor cel（lEPC） on Wister rats during experimental tooth movement.Methods 40 Wistar rats’teeth movement models were established and divided into experiment group and control group.After loading 1,3,5,7,14 days killed them respectively.The histological sections were stained with hematoxylin-eosin and rabbit anti-rat CD133 polyclonal antibody to express CD133 immunoreactivity.Results Expression of CD133 in the new vessels did not appear in control group.In the early experiment,the expression of CD133 was discovered in the new vascular endothelial cells of periodontium in experiment group.Expression of CD133 got the maximum after loading 1 day in experiment group,then decreased gradually,but it was not significantly higher than control group（P 0.05）.Conclusion CD133 ＋ EPC participated vascularized reaction in periodontal tissue of rat during the experimental tooth movement,direct participation was few and indirect effects possibly existed.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2010年第5期468-470,474,共4页
West China Journal of Stomatology
关键词
实验性牙移动
牙周组织
血管内皮祖细胞
CD133
experimental tooth movement
periodontal tissue
endothelial progenitor cell
CD133
