摘要
目的:建立灌流流速所决定的慢性各阶段肝损伤大鼠离体门脉压力方程.方法:常规观察CCl4慢性肝损伤第0、28、56、84天的肝脏系数与肝脏病,大鼠肝动脉、门脉和肝静脉插管,经肝动脉冲净残血,门脉灌流状态下游离肝脏;定量蠕动泵调节门脉灌流流速(μL/min),生物机能实验系统监测压力(mmHg),采用Prism-4非线型可变斜率回归由对数流速所决定的压力效能方程式,计算半效对数流速及其95%的可信区间.结果:在连续CCl4慢性肝损伤0、28、56、84天,由灌流数值流速(μL/min)决定的离体大鼠门脉灌流压力(mmHg)效能方程分别为y=-0.1428+1.1423/[1+10(13.805-3.84x)](r2=0.9996)、y=-0.1570+1.419/[1+10(4.666-1.27x)](r2=0.9958)、y=0.05106+0.96594/[1+10(10.877-2.958x)](r2=0.9987)和y=-0.09893+1.406/[1+10(4.3-1.158x)](r2=0.9989);半效对数流速(95%CI)分别为3.595(3.561-3.628)、3.674(3.285-4.064)、3.677(3.614-3.740)和3.713(3.449-3.977).结论:随着慢性肝损伤进展,离体门脉灌流流速所决定的压力方程具有不同系数和相同形式的特征,增高半效灌流流速可建立门脉舒张潜能低下的系统生物学门脉药效干模式.
AIM： To establish equations to define portal pressure based upon perfusion velocity in isolated chronically injured rat liver. METHODS： Chronic liver injury was induced in Wistar rats using carbon tetrachloride. The liver index and pathological changes were recorded. On days 0, 28, 56 and 84 after induction of chronic liver injury, the hepatic artery, portal vein and hepatic vein were cannulated. The residual blood in the isolated liver was eliminated with Krebs-Henseleit solution through the hepatic artery. Portal vein perfusion velocities were precisely controlled between 2 500 μL/min to 15 630 μL/min with a pump. The pressure （mmHg） was monitored using the BL-420S physiological experiment system. The equations for estimating portal pressure were developed using Prism-4 software, and median velocities and their 95% confidence intervals were calculated. RESULTS： On days 0, 28, 56 and 84, the equations for estimating portal pressure （mmHg） based on the logarithmic velocity （μL/min） in isolated rat liver were y = -0.1428＋1.1423/[1 ＋10（13.805-3.84x）] （r2 = 0.9996）, y = -0.1570＋1.419/ [1＋10（4.666-1.27x）] （r2 = 0.9958）, y = 0.05106＋0.96594 /[1＋10（10.877- 2.958x）] （r2 = 0.9987）, and y = -0.09893 ＋1.406/[1＋10（4.3-1.158x）] （r2 = 0.9989）, respectively. The median velocities （95% confidence intervals） were 3.595 （3.561-3.628）, 3.674 （3.285-4.064）, 3.677 （3.614-3.740） and 3.713 （3.449-3.977）, respectively. CONCLUSION： The equations for estimating portal pressure based on the logarithmic velocity in isolated rat liver are similar at different time points during the progression of carbon tetrachloride-induced chronic liver injury. A dry model of systems biology for portal pharmacodynamics could be derived from the increased median perfusion velocities.
出处
《世界华人消化杂志》
CAS
北大核心
2010年第26期2745-2749,共5页
World Chinese Journal of Digestology
基金
重大新药创制科技重大专项基金资助项目
No.2009ZX09502-017
国家重大基础研究基金资助项目
No.G2000056905
国家自然科学基金资助项目
No.30873464
教育部重点研究基金资助项目
No.108019~~
关键词
慢性肝损伤
离体灌流
门脉压力方程
大鼠
系统生物学
Chronic liver injury
Isolated perfusion
Portal pressure equation
Rat
Systems biology
