摘要
目的:探讨下调桩蛋白(paxillin)对人食管癌EC9706细胞中FAK及MMP-2表达的影响,并探讨其相关的分子机制.方法:将食管鳞癌EC9706细胞分为3组,即未处理组,对照siRNA组及paxillin siRNA组.利用paxillin siRNA和对照siRNA分别转染EC9706细胞,通过免疫细胞化学和Westernblot方法检测paxillin、FAK及MMP-2在3组细胞中的表达.结果:转染paxillin siRNA后,食管鳞癌EC9706细胞中paxillin蛋白表达显著下降,与未处理组及对照siRNA组相比,显著性具有差异(F=294.843,P=0.000).同时,paxillin siRNA组EC9706细胞中FAK及MMP-2表达也明显下降,3组间比较差异具有统计学意义(F=204.925,P=0.000;F=131.236,P=0.000).结论:Paxillin表达的下调能直接降低食管鳞癌中FAK及MMP-2的表达.
AIM：To investigate the effect of paxillin knockdown on the expression of focal adhesion kinase（FAK）and matrix metalloproteinase-2（MMP-2） proteins in esophageal squamous cell carcinoma（ESCC）EC9706 cells and to explore the potential molecular mechanisms involved.METHODS：EC9706 cells were divided into three groups：untreated group,control siRNA group,and paxillin siRNA group.Paxillin siRNA and control siRNA were transfected into EC9706 cells,respectively.The expression of paxillin,FAK and MMP-2 in EC9706 cells was detected by immunocytochemistry and Western blot.RESULTS：The expression of paxillin protein in the paxillin siRNA group was obviously decreased compared to the untreated group and control siRNA group（F=294.843,P=0.000）.The expression levels of FAK and MMP-2 proteins in the paxillin siRNA group were significantly lower than those in the untreated group and control siRNA group（F=204.925,P=0.000;F=131.236,P=0.000）.CONCLUSION：Down-regulation of paxillin expression decreases the expression of FAK and MMP-2 in EC9706 cells.
出处
《世界华人消化杂志》
CAS
北大核心
2010年第25期2626-2630,共5页
World Chinese Journal of Digestology
基金
河南省基础与前沿技术研究基金资助项目
No.082300453202~~
