摘要
本文利用脱氧核糖氧化法考察了飞灰颗粒物氧化能力与络合剂及盐酸浸取颗粒物上金属浓度的关系,结果表明,代表氧化损伤程度指标的脱氧核糖氧化物,通过硫代巴比妥酸似丙二醛加合物显色反应,其吸光度值与飞灰颗粒物被浸取剂盐酸及氨三乙酸作用后的金属离子铁、锌、镉、铅和锰的浓度显著相关,基于铁在自然环境中的高含量及在活性氧催化反应中的特殊性,本文认为二乙三氨五乙酸、乙二胺四乙酸及柠檬酸浸取液中的金属浓度不能很好地表征飞灰颗粒物对生物分子的氧化能力,颗粒物产生的生物氧化损伤可以被羟自由基清除剂所抑制。
By means of the deoxyribose oxidation assay that contains ascorbate, H2O2, coal or wood combustion fly ash, and deoxyribose, the relationship has been investigated between levels of oxi-dative injury by fly ash participates and the metal extraction concentrations with HCI and chelating agents, such as diethylenetriaminepentacetic acid (DTPA), ethylenediaminetetraacetic acid (ED-TA), nitrolotriacetate (NTA), and citric acid (GIT). The results demonstrate that, standing for the level of oxidative injuries, deoxyribose oxidized products, catalyzed by participates and measured as thiobaifoituric acid (TBA )-malondialdehyde (MDA) adducts, significantly correlate with iron, zinc, cadmium, lead, manganese with extractants 1.0 mol·l-1 HCI and 2.0 mol·l-1 NTA. For the reason of the natural abundance of iron and its propensity to catalyze reactive oxygen species, it is proposed that the extraction concentration with DTPA, EDTA, and CIT could not characterize the properties of oxidation associated with fly ash particulates. Scavengers dimethyl sul-foxide (DMSO) and mannitol inhibited the oxidized products of deoxyribose generated by fly ash.
出处
《环境化学》
CAS
CSCD
北大核心
1999年第3期221-226,共6页
Environmental Chemistry
基金
北京大学环境与污染控制国家重点联合实验室开放基金
关键词
活性氧
燃煤飞灰
燃木飞灰
浸取剂
氧化损伤
reactive oxygen species, coal combustion fly ash, wood combustion fly ash,metal, extractant.
