摘要
对猪伪狂犬病病毒(PRV)四川分离株(SL株)gK基因进行分子克隆,并利用生物信息学软件对gK基因进行了同源性、遗传进化树、密码子偏向性、蛋白质二级结构预测及抗原表位分析。结果显示,成功克隆了PRVSL株gK全基因,其编码区长939bp,编码313个氨基酸残基,与其他PRV分离株核苷酸序列同源性为97.6%～99.9%,与国外分离株的同源性略高于国内分离株。gK基因存在4个跨膜区及1个明显的CpG岛。表明成功克隆了PRVSL株gK基因,该基因具有潜在的抗原性。
gK gene of pseudorabies virus（PRV）SL strain was cloned.Based on the sequence,the homology,phylogeny tree,codon bias,secondary structure and B cell epitope were analyzed and predicted using bio-software.gK gene was successfully cloned and it consisted 939nucleotides encoding aprotein of 313amino acids.The gK gene shared 97.6%to 99.9%homology with that of the other PRV strains,which shared higher homology with that of the foreign isolates to the domestic isolates.The gK gene had four transmembrane regions and an apparent CpG island.The results showed that the gK gene of pseudorabies virus SL strain was cloned successfully and the gene has potential antigenicity.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2010年第10期1002-1006,共5页
Chinese Veterinary Science
基金
国家科技支撑计划项目(2006BAD06A18)
教育部"长江学者和创新团队发展计划"项目(IRT0848)
