摘要
目的:研究姜黄素对肺腺癌A549细胞增殖与凋亡的影响。方法:采用MTT法检测姜黄素对A549细胞存活率的影响。运用吖啶橙染色方法,观察细胞形态变化。利用TUNEL染色,检测DNA断裂情况。采用Western blot技术,检测P53蛋白表达变化。结果:姜黄素显著抑制A549细胞增殖。吖啶橙和TUNEL染色显示姜黄素可以诱导A549细胞凋亡。Western blot结果显示姜黄素提高了A549细胞中P53蛋白的表达。结论:姜黄素通过激活P53蛋白,抑制A549细胞增殖和诱导A549细胞凋亡。
Objective： To explore the effects of curcumin on the proliferation and apoptosis in A549 lung cancer cells.Methods： The cell viability was determined by MTT assay.Cell morphology and nuclear fragmentation were analyzed by acridine orange（AO） staining under fluorescent microscope.Cell apoptosis was detected by TUNEL assay.The content of P53 protein was analyzed by western blot assay.Results： Curcumin significantly inhibited A549 cell viability in a dose and time-dependent manner.The changes of morphology associated with apoptosis occurred when the cells were treated with 25 μg/mL and 50μg/mL curcumin for 48 h.When the cells were separately treated with 25μg/mL and 50μg/mL curcumin for 48 h,the relative content of P53 protein was significantly increased compared to control.Conclusion： The data suggested that curcumin might inhibit A549 lung cancer cell proliferation and induce apoptosis through activating P53.
出处
《中华中医药学刊》
CAS
2010年第10期2130-2132,共3页
Chinese Archives of Traditional Chinese Medicine
基金
山东省科技攻关项目(2006GG3202038)
山东省科学院博士基金项目(S2008YBS23)
