摘要
目的:观察葛根素对急性肺血栓栓塞溶栓治疗后P、E-选择素表达的影响。方法:24只日本大耳白兔,随机分为假手术组(S组)、单纯溶栓组(T组)、葛根素组(Pur组),经右心导管注入条柱状自体血栓,心导管法监测血流动力学,制备急性肺血栓栓塞实验模型。测定栓塞前、溶栓前即刻、溶栓后2h、4h血浆中超氧化物歧化酶(SOD)、丙二醛(MDA)的含量;采用免疫组化及原位杂交等方法观察肺微血管P、E-选择素表达的变化。结果:(1)T组和Pur组在溶栓开始后1h,PAMP即显著下降(P<0.01);在溶栓开始后1h Pur组PAMP即明显低于T组(P<0.05)。(2)与T组同期比较,Pur组在溶栓后2h、4h血浆SOD活力和MDA含量分别有明显升高和下降(P<0.05,P<0.01)。(3)Pur组与T组比较肺微血管P、E-选择素和P、E-选择素mRNA平均吸光度(А)值均有明显下降(P<0.05)。结论:葛根素可下调急性肺血栓栓塞溶栓后肺微血管P、E-选择素的表达,对溶栓治疗后再灌注损伤有保护作用。
Objective：To observe the effects of Puerarin on P-and E-selectins expression after thrombolytic therapy in acute pulmonary thromboembolism.Methods：Twenty-four Japanese rabbits were randomly divided into sham operation group（group S）,Thrombolysis-only group（group T）,and Puerarin group（group Pur）.Acute pulmonary thromboembolism models of rabbits were established with injection of autologous blood clots through the right heart catheters,haemodynamic monitoring were performed by introducing heart catheter through right jugular vein.The activity of plasma superoxide dismutase（SOD） and content of plasma malondialdehyde（MDA） were detected before embolization,before thrombolysis,2h and 4h after thrombolysis.The protein and mRNA expression of P-and E-selectins in microvessels were examined using immunohistochemistry and in situ hybridization.Results：（1） Pulmonary arterial mean pressure（PAMP） were decreased immediately at 1 hour after thrombolysis both in group T and group Pur（P〈0.01）,PAMP in group Pur were significantly lower than those in group T at 1h after thrombolysis（P〈0.05）.（2） At 2h and 4h after thrombolysis,the activity of SOD was higher（P〈0.05,P〈0.01） and the content of MDA was lower（P〈0.05,P〈0.01） in group Pur than those in group T.（3） The Аvalues of P E-selectin and P E-selectin mRNA in pulmonary microvessels were significantly lower in group Pur than those in group T（P〈0.05）.Conclusion：Chinese herb puerarin can protect lung against reperfusion injury after thrompolytic therapy in acute pulmonary thrombembolism which may associate with the action of inhibitting the expression P、E-selectin in pulmonary microvessels.
出处
《中华中医药学刊》
CAS
2010年第10期2118-2121,共4页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省中医药科技计划资助项目(2004C125)
关键词
葛根素
肺血栓栓塞
溶栓治疗
P-选择素
E-选择素
再灌注损伤
Puerarin
pulmonary thromboembolism
thrombolytic therapy
P-selectins
E-selectins
reperfusion injury
