摘要
目的 观察不同粒径二氧化钛（TiO2）对小鼠活性氧（ROS）水平的影响.方法 48只健康雄性昆明种小鼠随机分为对照组（四蒸水）、50 nm TiO2组（5 g/kg）、120 nm TiO2组（5 g/kg）.灌胃染毒后观察1周,用微波消解-电感耦合等离子体质谱（ICP-MS）法测定肝、肾、皮层及海马各组织中钛元素的含量,用流式细胞仪测定各组织细胞中ROS的含量.结果 各组小鼠一般状况良好,饮水量、进食量未见明显改变,体重增重及肝、肾、脑的脏器系数与对照组的差异无统计学意义（P〉0.05）.50、120 nm TiO2处理后,小鼠肝、肾、皮层、海马各组织中Ti含量明显高于对照组,差异有统计学意义（P〈0.05）;且50 nm处理组的Ti含量明显高于120 nm处理组,差异有统计学意义（P〈0.05）;50、120 nm TiO2组小鼠肝脏、肾脏和皮层组织细胞中ROS水平（273.2±32.5、160.2±28.5、74.9±8.9,159.4±15.9、64.4±7.5、41.2±5.6）与对照组（74.9±6.4、24.9±2.8、32.8±3.1）相比明显升高;50 nm处理组小鼠海马细胞中ROS水平与对照组相比明显升高,差异均有统计学意义（P〈0.05）;且50 nm组各组织中ROS水平明显高于120 nm组,差异亦有统计学意义（P〈0.05）.结论 不同粒径TiO2经口急性染毒后Ti元素可以分布于小鼠肝、肾、脑组织中,使小鼠肝脏、肾脏、皮层和海马细胞中ROS生成增加,且 50 nm TiO2的作用明显大于120nm TiO2.
Objective To observe the effects of different sized titanium dioxide on reactive oxygen species （ ROS ） levels. Methods Forty-eight healthy mice were divided into 3 groups randomly： control group （distilled water）, 50 nm TiO2 （5 g/kg） group and 120 nm TiO2（5 g/kg） group. The mice were exposed to distilled water or TiO2 by a syringe via gastrointestinal tract in a minute. One week later, the mice were sacrificed and the liver, kidney and brain（cortex, hippocampus） were collected. The contents of Ti in the above tissues of mice were measured by ICP-MS. The ROS levels in the tissues were measured by flow cytometry. Results There was no significant difference in organ coefficient between two exposure groups and control group （P〉0.05）. The Ti contents in liver, kidney, cortex and hippocampus of two exposure groups were higher than those of control group （P〈0.05） ;The Ti contents of above four tissues in 50 nm TiO2 group were higher than those in 120 nm group （P〈0.05）. The ROS levels in liver, kidney and cortex cells of two exposure groups （273.2±32.5, 160.2±28.5, 74.9 ±8.9; 159.4 ± 15.9, 64.4 ±7.5, 41.2 ±5.6 ） significantly increased, as compared with control group （74.9±6.4,24.9±2.8,32.8±3.1） （P〈0.05）. The ROS levels in hippocampus cells in 50 nm TiO2 group were significantly higher than those in control group （P〈0.05）. The ROS levels of above four tissues in 50 nm group were significantly higher than those in 120 nm TiO2 group （P〈0.05）. Conclusion After mice were exposed to 50 nm and 120 nm TiO2 particles via gastrointestinal tract, Ti could be distributed into the liver, kidney and the brain tissues in mice, resulting in the enhanced ROS levels in liver, kidney, cortex and hippocampus cells.Moreover, the ROS levels induced by 50 nm TiO2 particles are significantly higher than those induced by 120 nm TiO2 particles.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2010年第9期664-666,共3页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
河北省自然科学基金资助项目（C2009001073）
河北省教育厅自然科学基金资助项目（2008131）
河北省卫生厅科研基金资助项月（08059）
关键词
钛
活性氧组分
脂质过氧化作用
Titanium
Reactive oxygen species
Lipid peroxidation
