摘要
目的分析比较目前常用的几种检测抗ＥＮＡ抗体方法的敏感性和特异性。方法用免疫印迹法（ＩＢＴ）、酶免法（ＥＬＩＳＡ）及对流免疫电泳法（ＣＩＥ）检测１３０例各种结缔组织病、非结缔组织病人及健康人血清中的抗ＥＮＡ抗体。结果２０例健康人的血清三种方法检测均为阴性；８０例结缔组织病患者，检测抗Ｓｍ抗体、抗ＲＮＰ抗体，ＩＢＴ法、ＥＬＩＳＡ法均较ＣＩＥ法敏感（Ｐ＜００５）。检测抗ＳＳＢ抗体，ＥＬＩＳＡ法较ＣＩＥ法、ＩＢＴ法敏感（Ｐ＜００５）。抗ＳＳＡ抗体的检测，ＩＢＴ法由于采用兔胸腺丙酮粉为抗原，ＳＳＡ抗原成分有缺失，造成检出率低。ＩＢＴ法与ＥＬＩＳＡ法检测的符合率较高。结论ＩＢＴ法、ＥＬＩＳＡ法检测抗Ｓｍ抗体、抗ＲＮＰ抗体时，敏感性优于ＣＩＥ法。ＣＩＥ法、ＥＬＩＳＡ法检测抗ＳＳＡ抗体优于ＩＢＴ法。ＥＬＩＳＡ法检测抗ＳＳＢ抗体敏感性较高。上述三种方法在临床抗ＥＮＡ抗体的检测中可互相验证和补充。
Objective To analyse and compare the sensitivity and specificity of the common
methods used now in the detection of antiENA antibodies. Methods All 130 serum samples from
80 patients with different connective tissue diseases(CTD),30 with non CTD patients and 20
from normal volunteers were collected and studied.These sera were tested for antiSm,antiRNP,
antiSSA and antiSSB antibodies with ELISA,immunoblot test (IBT) and current
immunoelectrophoresis(CIE) methods. ResultsZThe sensitivity of antiSm and antiRNP
antibodies was augmented with IBT and ELISA,such as the detection of antiSSB antibodies with
ELISA.Because the antigens were acetone that come from hares chest gland and then the SSA
antigens were lacking and sensitivity of antiSSA antigens was lacking,so the sensitivity of
antiSSA antibodies was the lowest with IBT.IBT was in accord with ELISA. Conclusion It is
suggested that three methods may be complementary in detection of antiENA antibodies.
出处
《中华风湿病学杂志》
CAS
CSCD
1999年第2期92-94,共3页
Chinese Journal of Rheumatology
关键词
抗ENA抗体
ELISA
免疫印迹法
对流免疫电泳
ntiENA antibodyEnzymelinked immunosorbent assayImmunoblottingCounter
immunoelectrophoresis
