摘要
目的:为临床诊断乙型肝炎提供灵敏、准确、可靠的实验依据。方法:采用PCR杂交梳(反相膜杂交法),对71例乙型肝炎患者血清进行DNA验证性检测,并与一般聚合酶链反应(PCR琼脂糖凝胶电泳法)同步对比分析。结果:71例乙型肝炎患者血清中杂交梳方法检出HBV DNA阳性58例,阳性率81.7％;琼脂糖凝胶电泳质检出HBV DNA阳性50例,阳性率70％。结论:PCR杂交梳法较PCR法具有更高特异性和敏感性。
Aim:To probe into a more sensitive.more accurate and more specific method to detect HBV DNA. Methods: Serum samples of 71 patients with hepatitis B were detected for HBV DNA by PCR hybri-comb (reversal membrane hybridization), at the same time, usual polymerase chain reaction assay (PCR-electrophoresis in agarose gel) was given as controls. Result: 58 serum samples were positive for HBV DNA by PCR hybri-comb assay among 71 patients samples. Positive ratio was 81. 7% ; however, 50 serum samples were positive by PCR-electrophoresis in agarose gel, and positive ratio was 70%. Conclusion: PCR hybri-comb technique is satisfactory with sensitivity and specificity in detecting HBV DNA.
出处
《中西医结合肝病杂志》
CAS
1999年第3期8-9,共2页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
