摘要
目的:研究丹参素钠对大鼠脑缺血再灌注(I/R)损伤的保护作用。方法:成年Wistar大鼠50只随机分为模型对照组、低剂量组、中剂量组、高剂量组、阳性对照组,MCAO法建立大鼠脑缺血模型,脑缺血1h,再灌注24h后进行神经缺损症状行为评分;TTC染色法观察脑梗死面积;比色法测定组织总超氧化物歧化酶(t-SOD)活力、谷胱甘肽过氧化物酶(GSH-PX)活力、黄嘌呤氧化酶(XOD)活力、丙二醛(MDA)含量。结果:与模型对照组相比,低剂量组、中剂量组、高剂量组和阳性对照组缺血1h再灌注24h后行为评分降低(P<0.01);脑梗死范围显著减小;t-SOD活力升高(P<0.01);GSH-PX活力升高(P<0.01);XOD活力降低(P<0.01);MDA含量降低(P<0.01)。结论:丹参素钠可以降低脑I/R后对神经的损伤,减小脑梗死范围,提高脑组织抗氧化能力,减少内皮功能降低,从而降低脑I/R对脑组织的损伤。
OBJECTIVE To investigate the effect of sodium salvianic acid A in protecting cerebral tissue from ischemia/ reperfusion injury. METHODS Fifty adult Wistar rats were randomly divided into model control group, low dose group, middie-dose group, high-dose group of sodium salvianic acid A, and the positive control group. The model of focal cerebral ischemia/reperfusion was established in rats by occluding the middle cerebral artery with a nylon thread. Neurological deficit was evaluated 24 h after I/R and cerebral infarction size was determined by TTC staining. Activities of total-superoxide dismutase （t-SOD）, glutathione peroxidase （GSH-PX）, xanthine oxidase （XOD）, content of malonaldehyde （MDA） in cerebral tissue were determined by spectrophotometer. RESULTS After 24h of reperfusion, compared with model control group, the neurological scores（P〈0. 01 ） and infarction size of cerebral of low dose group, middle-dose group, high-dose group and the positive control group decreased; the activities of t-SOD （P〈0. 01） and GSH-PX （P〈0. 01） increased, the activities of XOD （P〈0. 01） and the content of MDA decreased （P〈0. 01）. CONCLUSION Sodium salvianic acid A could decrease the neurological deficit and infarction size of cerebrum, increase the ability of anti-oxidation after I/R, reduce the cerebral isehemia-reperfusion injury of brain tissue, thereby protect cerebral tissue from injury of I/R.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2010年第18期1545-1549,共5页
Chinese Journal of Hospital Pharmacy
关键词
丹参素钠
脑缺血再灌注损伤
大脑中动脉闭塞
脑梗死
抗氧化
sodium salvianic acid A
cerebral ischemia/reperfusion injury
MCAO
cerebral infraction
antioxidation
