摘要
目的探讨人工关节腐蚀磨损等产生的金属离子钴(Co2+)、铬(Cr3+)对人工关节无菌性松动的影响,以寻求防治假体周围骨溶解的方法。方法体外培养小鼠单核/巨噬细胞(RAW264.7),用Co2+、Cr3+分别干预单核/巨噬细胞,将细胞分为3组:阴性对照组为单纯单核/巨噬细胞,铬离子组为单核/巨噬细胞+500mg/LCr3+,钴离子组为单核/巨噬细胞+10mg/LCo2+。分别在12h、24h、48h用MTT方法检测三组细胞活性及用半定量逆转录-聚合酶链反应(RT-PCR)方法测定单核/巨噬细胞表面RANKmRNA的表达量。结果 MTT显示与阴性对照组相比,钴离子组和铬离子组中的单核/巨噬细胞在12h、24h、48h的细胞活力明显下降。(RT-PCR)方法测定钴离子组和铬离子组中的单核/巨噬细胞RANKmRNA与阴性对照组相比,在12小时表达均增强(<0.05),24小时均达到高峰(<0.05),48小时相对24h表达都有所下降(﹤0.05)。结论钴离子和铬离子对单核/巨噬细胞有细胞毒性,且能够诱导单核/巨噬细胞RANKmRNA的表达,从而加重假体周围骨溶解和假体松动。
Objective Discuss the effect of cytotoxicity of cobalt（Co2＋） and chromium（Cr3＋） Produced by corrosion wear of artificial joint to aseptic loosening,to search a promising therapeutic candidate for the prevention and treatment of aseptic loosening.Methods Culture monocyte-macrophage cells in vitro.Monocyte-macrophage cells exposed to Co2＋ and Cr3＋ ions,The cells were divided into 3 groups,Negative control group：monocyte /macrophages;Chromium ion group：monocyte /macrophages ＋ 500 mg/L Cr3＋ ;Cobalt ion group：monocyte /macrophages ＋ 10 mg/L Co2＋;MTT test demonstrated that Co2＋and Cr3＋ ions can both obviously decrease the cell viability of Monocyte-macrophage cells At 12h,24h,48h.Cells exposed to Co2＋ and Cr3＋ ions,compared to the control and the some metal ions group compared to each other at different times,mRNA expression of RANK was incresaed at 12h and reached its peak level at 24h and also reduced at 48h Compared 24h in vitro.Conclusion Co2＋、Cr3＋ ions have a cytotoxic effect on Monocyte macrophage cells and can stimulate the expression of RANK in Monocyte-macrophage cells.So it can increase the periprosthetic osteolysis and prosthetic loosening.
出处
《生物骨科材料与临床研究》
CAS
2010年第5期16-19,共4页
Orthopaedic Biomechanics Materials and Clinical Study
基金
国家自然科学基金(30760255)
