摘要
将伯氏疟原虫感染小鼠，当其血原虫密度大于３０－００ ％ ，置病鼠于－ ２０ ℃低温冰箱保存。经不同时间后，迅速解冻，心脏取血，经腹腔内接种健康小鼠，以观察原虫复苏成功率。结果表明：低温保存５０ ～１００ ｄ 和１０１～１５５ ｄ，病鼠复苏成功率分别为３３－３３ ％ 和２０－００ ％ 。提示该法仍不适为一种简便可行，行之有效的低温保存方法。
When parasitemia in infected mice with plasmodium berghei exceeded 30.00%,the diseased mice were frozen at -20℃.At different times,the frozen mice were thrawed and bleed from the heart quickly.The blood was inoculated into the healthy mice by the method of abdominal cavity injection so as to observe the successful rate of resuscitation.The result shows that the rate of resuscitation reached 33.33% and 20.00% respectively,when the diseased mice were frozen 50～100 d and 101～155 d at -20℃,We conclude that the method was a simple,convenient and efficient low temperature preservation.
