摘要
目的:建立以高效液相色谱电喷雾串联质谱(LC-MS/MS)法测定人血浆中甲磺酸雷沙吉兰浓度的方法,并研究其在健康人体内的药动学。方法:以盐酸罂粟碱为内标,血浆样品经液-液萃取后,采用Intersil ODS-3进行分离,通过三重四级杆串联质谱仪,以选择性反应监测(SRM)方式进行测定。结果:甲磺酸雷沙吉兰血药浓度在0.010～40ng·mL-1范围内线性关系良好(r=0.9998);方法回收率为90.55%～101.57%,日内、日间RSD均≤15%。结论:本方法快速、准确、灵敏,适用于甲磺酸雷沙吉兰的人体药动学研究。
OBJECTIVE：To establish an LC-MS/MS method for the determination of rasagiline mesilate in human plasma and study its pharmacokinetics in healthy subjects.METHODS：Plasma samples were extracted using liquid-liquid extraction with cardoverine as internal standard then separated on Intersil ODS-3 column.The content of rasagiline mesilate in human plasma was detected by selective reaction monitoring（SRM）on a triple quadrupoles tandem mass spectrometer.RESULTS：The linear range of rasagiline mesilate was 0.010～40 ng·mL-（1r=0.999 8）with an recovery rate of 90.55%～101.57%.The RSD of inter-day and intra-day were all lower than 15%.CONCLUSION：The method is rapid,accurate and sensitive for pharmacokinetic study of rasagiline mesilate.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第38期3598-3600,共3页
China Pharmacy
