摘要
目的探讨草珊瑚不同萃取部位的抗炎作用。方法采用系统溶剂法按极性由低到高分离得到草珊瑚石油醚萃取物、乙酸乙酯萃取物、正丁醇萃取物、水提取物4个部位,建立RAW264.7细胞炎症模型,对各部位抑制细胞炎症因子NO表达进行研究,通过MTT法测定各部位对RAW264.7细胞增殖的影响及评价细胞毒性分极。结果通过RAW264.7炎症模型的筛选,草珊瑚乙酸乙酯部位(100～200μg/mL)和多糖部位(100～400μg/mL)均可显著抑制模型细胞NO的表达(P<0.01),并可显著抑制RAW264.7细胞增殖水平,且其细胞毒性分级均为1级或以下。结论草珊瑚多糖和乙酸乙酯部位是草珊瑚抗炎作用的主要活性部位。
Objective To investigate the anti-inflammatory effect of different extract parts of Sarcandra glabra （Thtmb.） Nakai. Methods Separated the ethanol extract of Sarcandra glabra （Thunb.） Nakal and got four parts by solvent extraction. RAW264.7 cell models were established to study the inhibitory effect of different extract parts on expression of cell inflammatory factor, and the effects of each part on proliferation of RAW264.7 cell were observed by MTT methods and cytotoxicity were determined by RGR levels. Results Ethyl acetate and polysaccharides fraction could get definite and clear results. Both of these two parts could significantly inhibit NO expression （P〈0.01）, also significantly inhibit the proliferation of RAW264.7 cell, and they both showed relatively lower cytotoxicity （0 or 1 grade）. Conclusion Ethyl acetate and polysaccharides fraction are main active sites of anti-inflammatory effect of Sarcandra glabra （Thunb.） Nakai.
出处
《福建中医学院学报》
2010年第5期35-38,共4页
Journal of Fujian College of Traditional Chinese Medicine
基金
福建省卫生厅重点项目(WZZB0903)
关键词
草珊瑚
抗炎活性
增殖
有效部位
炎症细胞模型
Sarcandra glabra （Thunb.） Nakai
anti-inflammatory
proliferation
active sites
models of inflammatory cell
