摘要
目的 观察活化T细胞核因子-2（NFAT2）在高迁移率族蛋白B1（HMGB1）促进白细胞介素-2（IL-2）转录表达中的介导作用.方法 将构建好的HMGB1和NFAT2质粒单独或共同转染人胚胎肾细胞株293T,同时转染IL-2报告基因,并在此基础上应用小分子干扰RNA（siRNA）质粒对内源性及外源性NFAT2表达进行特异性抑制,观察对IL-2报告基因活性的影响.结果 在293T细胞中,内源性转染实验促使IL-2报告基因活性增加到17.81±1.49,但随着siRNA质粒转染剂量从0 μg/孔逐渐增加到4.8μg/孔,IL-2报告基因活性在内源性抑制实验中降低最小到1.42±0.17,在外源性抑制实验中活性最高为54.16±5.49,受抑制后降低最小到2.97±0.34.结论 NFAT2介导HMGB1促进IL-2报告基因转录表达.
Objective To evaluate nuclear factor of activating T cells 2 （ NFAT2）-mediated transcription and expression of interleukin （IL）-2 induced by high mobility group box-1 protein （HMGB1）.Methods HMGB1 or NFAT2 plasmids were transfected into 293T cells respectively or simultaneously.Doses of small RNA interfering （siRNA） for NFAT2 were increased from 0 to 4.8 μg/plate steadily and reporter gene activity of IL-2 was detected and compared. Results Reporter gene activity of IL-2 was increased to 17.81 ± 1.49 after HMGB1 plasmid transfection, and decreased to 1.42 ± 0.17 by transfection of 4.8 μg/plate siRNA plasmid for NFAT2 in endogenous inhibition examination. Reporter gene activity of IL-2 was increased to 54.16 ± 5.49 after HMGB1 and NFAT2 plasmids transfection, and decreased to 2.97± 0.34 by transfection of 4.8 μg/plate siRNA plasmid for NFAT2 in exogenous inhibition examination.Conclusion NFAT2 plays an important role in transcription and expression of IL-2 induced by HMGB1.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2010年第10期1425-1428,共4页
Chinese Journal of Experimental Surgery
基金
基金项目：国家自然科学基金资助项目（30801187、30872683、30672178）
国家重点基础研究发展规划项目（2005CB522602）
