摘要
目的测定大白鼠肠道缺血再灌注损伤后肺表面活性物质（ＰＳ）的浓度及活力，结合肺组织病理改变，探讨急性肺损伤的病理机制。方法健康雄性ＳＤ大白鼠随机分三组：Ａ组（对照）；Ｂ组（肠缺血６０分钟）；Ｃ组（肠缺血６０分钟再灌注１２０分钟）。右肺灌洗后，测灌洗液中总蛋白（ＴＰ）、总磷脂（ＴＰＬ）、饱和磷脂（ＤＳＰＣ）浓度；测左肺上叶干／湿比值；左肺下叶病理检查。结果１、肠道缺血再灌注后肺泡灌洗液中蛋白质含量显著升高（Ａ组与Ｃ组比较Ｐ＜０．０１）；再灌注后总磷脂含量明显低于对照组（Ｐ＜０．０１）。２、肺干／湿比值：缺血组较对照组明显降低Ｐ＜０．０５；再灌注组较对照组降低更为显著Ｐ＜０．０１。３、病理检查：Ｂ组：肺组织内毛细血管充血，间质增宽，水肿，炎症细胞渗出。Ｃ组：肺组织内毛细血管充血，间质增宽，水肿，炎症细胞渗出，部分肺泡不张，肺泡水肿，肺出血。结论肠道缺血再灌注可引起急性肺充血、水肿肺泡不张和肺出血等损伤；在急性肺损伤中，肺表面活性物质的含量明显降低及活力的明显减弱可能为肺部病理改变的原因。
Objective To determine the pulmonary surfactant changes in
acute lung injury of adult rats with ischemia reperfusion of intestine. Methods Healthy S D
rats (bw 200～250g) were anesthetized and divided into 3 groups: A group as control; B group
with SMA occlusion for 60min and C group with intestinal ischemia for 60min and reperfusion for
120min. The left lung was used for measurements of dry/wet weight ratio (D/W) and
histopathology, whileas the right lung was lavaged and determined the total proteins (TP),
disaturated phosphatidylcholine (DSPC), and total phospholipids (TPL) in the lavage fluid.
Results 1. The values and activities of PS: TP:The TP were higher after intestinal ischemia ( P
< 0.01) and were obviously higher after intestinal ischemia reperfusion. ( P CvsA <0.001;
P CvsB <0.05). TPL: The values of TPL and DSPC after ischemia reperfusion were lower
than those in the control ( P < 0.01). 2. There was pulmonary edema after intestinal ischemia
and being more severe after reperfusion. (D/ W P BVsA <0.05; P CVsA <0.01) 3.
Pathological examination: There was engorgement in pulmonary vessels, interstitial edema
and inflammatory cellular infiltration in group B and group C. But in group C, hemorrhage and
atelectasis were also present.Conclusion I/ R resulted in severe lung injuries, as seen by
decrease of DSPC and increase of protein in alveolar space which may lead to a secondary
surfactant deficiency and pulmonary dysfunction.
出处
《上海医学》
CAS
CSCD
北大核心
1999年第4期207-209,共3页
Shanghai Medical Journal
基金
上海市"曙光计划"和"百人培养计划"资助
