摘要
以人工合成的微卫星序列（ＧＴＧ）５、（ＧＴ）８、（ＣＡＣ）５和人源小卫星３３．１５做引物，首次随机扩增了伏翼的基因组ＤＮＡ，从多态性ＤＮＡ片段中回收８条特异性片段，即（ＧＴＧ）５ａ、（ＧＴＧ）５ｂ、（ＧＴ）８ａ、（ＧＴ）８ｂ、（ＣＡＣ）５ａ、（ＣＡＣ）５ｂ、３３．１５ａ和３３．１５ｂ．与被标记过的基因组ＤＮＡ做探针斑点杂交结果表明，８个片段中（ＧＴＧ）５ａ、（ＣＡＣ）５ａ和３３．１５ａ产生了阳性信号，因此，这三个片段被标记后做探针可用于伏翼ＤＮＡ指纹谱的制备．
The DNA polymorphic fragments of Pipistrellus abramus were first amplified by the polymerease chain reaction (PCR) with artificial primers that contained (GTG)5,(GT)8,(CAC)5 and human minisatellite 33.15. Eight bands were retrieved among the DNA electrophoresis polymorphic bands.it were (GTG)a、(GTG)b、(GT)a、(GT)b、(CAC)a、(CAC)b、33.15a and 33.15b.The results of dot hybridization showed that, When the eight fragments and Genomic DNA as the probes were hybridized,(GTG)a、(CAC)a and 33.15a could emerge positive signals.It means that the three fragments labeled could be applied in the analysis of Pipistrellus abramus DNA fingerprint.
出处
《曲阜师范大学学报（自然科学版）》
CAS
1999年第3期89-92,共4页
Journal of Qufu Normal University(Natural Science)
关键词
伏翼
DNA指纹谱
探针
筛选
Pipistrellus abramus
DNA fingerprint
probe
