摘要
目的:从正常女性生殖道中分离乳杆菌,为开发复方益生菌制剂提供新型菌株。方法:采用传统方法鉴定出乳杆菌,进一步筛选出产生过氧化氢的优势菌株,并运用分子生物学方法,对其进行基因型鉴定。并对优势菌进行初步的性能鉴定试验。结果:从18名体检女性阴道分泌物内分离纯化得到11株菌,检出率为61.1%。分别为嗜酸乳杆菌4株,格氏乳杆菌3株,詹氏乳杆菌2株,卷曲、唾液乳杆菌各1株。结论:运用传统的细菌学分离方法与分子生物学对分离的菌株进行了鉴定。其中一株为产生过氧化氢的卷曲乳杆菌,经过初步测试,该菌株具有良好的耐酸、耐高温及体外拮抗其他病原微生物的能力。
Objective：Isolating lactobacillus from health female genital tract, for exploring probiotics to research compound preparation.Methods：Traditional morphology analysis was used to identify lactobacillus, and further screen hydrogen peroxide-producing lactobacillus.The molecular biology method was used to identify exactly and to examine predominant strain by performance test.Results：There were 11 strains of lactobacillus isolated and identified from 18 health examination women genital tract, the detection rate was 61.1%.According to the results of these experiment,11 strains were identified that 4 were lactobacillus acidophilus, 3 were lactobacillus gasseri, 2 were lactobacillus jensenii,1 was lactobacillus crispatus and another one was lactobacillus salivarius.Conclusion：Traditional biochemical identification and molecular biology methods could identify lactobacillus.Only lactobacillus crispatus is hydrogen peroxide-producing one. After the preliminary test, the strain is a strong acid-resistant and hot-resistant one, and is bacteriostasis in vitro testing.
出处
《中国妇幼保健》
CAS
北大核心
2010年第27期3950-3953,共4页
Maternal and Child Health Care of China
基金
西北民族大学中青年科研基金项目〔XBMU-2008-BD-95〕
