摘要
为更好地提高青贮饲料的质量,降低微生物接种剂的生产成本,本研究对采集自德国青贮窖中的主导菌群中一株乳酸菌GLP01进行了分子学鉴定,并对培养条件进行了优化。通过单因素试验设计研究培养基组成(碳源、氮源)和培养条件(温度、接种量、起始pH等)对GLP01乳酸菌生长繁殖的影响;采用二次响应面分析方法对GLP01培养条件进行优化,得到GLP01生长模型,以及取得模型最优值时各因素的水平。单因素试验结果表明,GLP01的最适碳源是果糖,最佳氮源是酵母粉;二次响应面分析确定的GLP01最佳培养条件是起始pH5.47、培养温度35.3℃、接种量8.16%。结果提示,乳酸菌GLP01可以作为微生物接种剂制作青贮饲料,但青贮效果仍有待研究。
To improve the quality of silage and reduce the cost of microbial inoculants,molecular biological assay was carried out on a major strain of lactic acid bacteria called GLP01,which was isolated from a silage cellar in Germany,and the culture conditions were optimized.One-factor-at-a-time approach was used to investigate the effects of different medium components （carbon source and nitrogen source） and different culture conditions （temperature,initial pH,and inoculum size） on growth of GLP01;response surface technique （RST） was applied to the optimization of culture condition to get the growth model and factor levels of the optimal model value.The results showed as follows：the optimal carbon source and nitrogen source were fructose and yeast powder,respectively;the optimal culture conditions of GLP01 were pH 5.47,temperature 35.3 ℃,and inoculum size 8.16%.In conclusion,lactic acid bacteria GLP01 can be used as silage microbial inoculants,but the effect remains to be clarified.
出处
《动物营养学报》
CAS
CSCD
北大核心
2010年第5期1307-1313,共7页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家“十一五”科技支撑项目(2006BAD04A04-05)
关键词
青贮饲料
乳酸菌
培养基优化
响应面法
silage
lactic acid bacteria
culture medium optimization
response surface technique
