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靶向c-myc基因小RNA干扰对胆囊癌细胞侵袭运动的影响 被引量：4

Influence of c-myc-targeted siRNA on migration and invasion abilities of gallbladder cancer cells
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摘要 目的研究c-myc基因在胆囊癌侵袭和转移中的作用及其机制。方法采用实时定量PCR和蛋白质印迹法检测亲代和高转移胆囊癌细胞系中c-myc基因表达,设计靶向c-myc的干扰性小RNA(siRNA)转染高转移的胆囊癌细胞,体外Transwell实验研究c-myc siRNA对胆囊癌高转移细胞迁移和侵袭能力的影响。通过蛋白质印迹法检测转染c-myc siRNA后c-myc下游部分信号通路的变化。结果高转移细胞中c-myc基因表达高于亲代细胞,转染c-mycsiRNA后,c-myc的表达在转录和翻译水平均降低。同时GBC-SD/M3细胞的转移和侵袭能力受到抑制。对c-myc下游信号通路的检测发现LIN28的表达下调。结论 c-myc在高转移的胆囊癌细胞中表达上调;通过siRNA下调其表达后可以抑制胆囊癌细胞的转移和侵袭,这种抑制作用可能与LIN28的下调有关。
作者 蔡民 李立波
机构地区 浙江大学医学院附属邵逸夫医院普通外科
出处 《第二军医大学学报》 CAS CSCD 北大核心 2010年第9期1029-1031,共3页 Academic Journal of Second Military Medical University
关键词 C-MYC 胆囊肿瘤 肿瘤转移 RNA干扰 LIN28 LET-7
分类号 R735.8 [医药卫生—肿瘤]
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参考文献11

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二级参考文献6

共引文献7

同被引文献26

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二级引证文献7

第二军医大学学报
2010年 第9期

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